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- W2079974911 abstract "A human genomic clone, psi GS, containing an intron-less glutamine synthetase (GS)-encoding pseudogene, was isolated by screening a human library. A sequence of 3004 bp, containing the GS coding region and both the 5' and 3' flanking sequences, was identified that exhibits all the characteristics of a processed pseudogene. The coding region shows 93% identity with the human GS cDNA (hGS) sequence and contains two frame-shifts and two termination codons. The coding sequence is flanked by a 9-bp AT repeat and a putative polyadenylation site, AATAAA, at the 3' end. Primer extension analysis and S1 nuclease mapping showed a transcription start point (tsp) 62 bp upstream from the start codon indicating a shorter untranslated region than hGS. Transfection of HeLa cells with cat constructs containing portions of the 5' flanking sequence showed the presence of a functional promoter/enhancer within 200 bp of the tsp, independent of its orientation." @default.
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- W2079974911 date "1995-02-01" @default.
- W2079974911 modified "2023-09-27" @default.
- W2079974911 title "Detection of a functional promoter/enhancer in an intron-less human gene encoding a glutamine synthetase-like enzyme" @default.
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- W2079974911 doi "https://doi.org/10.1016/0378-1119(94)00751-d" @default.
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