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• W2079981775 abstract "Tissue engineering requires growth factors, cells and a scaffold to permit effective tissue regeneration. This study aimed to develop a scaffold with a focus on immobilizing growth factors within gelatin. We focused on the extracellular matrix and developed a heparin-conjugated gelatin (Hep-gela). Conjugation was confirmed using the alcian blue assay and X-ray diffraction patterns. The mechanical strength and stability of the Hep-gela gel in protease solution were improved compared with collagen gel. Hep-gela was able to immobilize vascular endothelial growth factor (VEGF) even in the presence of albumin, with an efficiency of 54.2%. Immobilized VEGF promoted proliferation of human umbilical vein endothelial cells. Hep-gela-immobilized VEGF maintained its native biological activity. In summary, Hep-gela has the potential to become an effective material in the field of regenerative medicine." @default.
• W2079981775 created "2016-06-24" @default.
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• W2079981775 date "2013-05-01" @default.
• W2079981775 modified "2023-10-03" @default.
• W2079981775 title "Heparin-conjugated gelatin as a growth factor immobilization scaffold" @default.
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• W2079981775 doi "https://doi.org/10.1016/j.jbiosc.2012.11.011" @default.
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