Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080151330> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2080151330 endingPage "1110" @default.
- W2080151330 startingPage "1099" @default.
- W2080151330 abstract "1,25-Dihydroxy-16-ene vitamin D3 is a synthetic analog of 1,25-dihydroxyvitamin D3, the most physiologically active metabolite of vitamin D3. The renal metabolism of 1,25-dihydroxy-16-ene vitamin D3 had been studied previously using a perfused rat kidney system [Reddy et al., Bioorg Med Chem Lett 3: 1879-1884, 1993], and its C-24 oxidative metabolic pathway had been found to be different from that of 1,25-dihydroxyvitamin D3 by HPLC. To further delineate the differences between the C-24 oxidative metabolic pathways of 1,25-dihydroxyvitamin D3 and 1,25-dihydroxy-16-ene vitamin D3 in this present study we investigated the C-24 oxidation pathway of 1,25-dihydroxy-16-ene vitamin D3 using a novel detection approach based on on-line capillary liquid chromatography coupled to electrospray tandem mass spectrometry. Two types of tandem mass spectrometric detection were employed to characterize the metabolites in the kidney perfusate: (a) the preliminary screening of metabolites by parent scan, which led to the tentative discovery of the production of 1,23,25-trihydroxy-24-oxo-16-ene vitamin D3, a new metabolite of 1,25-dihydroxy-16-ene vitamin D3, and (b) the pharmacokinetic studies of the substrate, 1,25-dihydroxy-16-ene vitamin D3 and its metabolites by multiple reaction monitoring. In the latter, the mass spectrometric sensitivity for quantification was found to be about 20-fold better than UV detection. The current work concluded that the C-24 oxidative metabolic pathway of 1,25-dihydroxy-16-ene vitamin D3 closely mimicked that of its natural counterpart. Furthermore, the use of mass spectrometry permitted the clearance rate of the starting substrate to be studied at a more physiological level (ng/mL or submicromolar level), which had not been possible previously by HPLC-UV detection." @default.
- W2080151330 created "2016-06-24" @default.
- W2080151330 creator A5007758316 @default.
- W2080151330 creator A5043268325 @default.
- W2080151330 creator A5044130061 @default.
- W2080151330 creator A5085298324 @default.
- W2080151330 date "1995-04-01" @default.
- W2080151330 modified "2023-09-26" @default.
- W2080151330 title "Characterization of the metabolic pathway of 1,25-dihydroxy-16-ene vitamin D3 in rat kidney by on-line high performance liquid chromatography-electrospray tandem mass spectrometry" @default.
- W2080151330 cites W1854781184 @default.
- W2080151330 cites W1977960826 @default.
- W2080151330 cites W2000935329 @default.
- W2080151330 cites W2022710508 @default.
- W2080151330 cites W2030965046 @default.
- W2080151330 cites W2038083315 @default.
- W2080151330 cites W2043232638 @default.
- W2080151330 cites W2049284566 @default.
- W2080151330 cites W2060536706 @default.
- W2080151330 cites W2065897358 @default.
- W2080151330 cites W2077420287 @default.
- W2080151330 cites W2080060312 @default.
- W2080151330 cites W2094493372 @default.
- W2080151330 cites W2094523739 @default.
- W2080151330 cites W2163124188 @default.
- W2080151330 cites W2163882073 @default.
- W2080151330 cites W331682844 @default.
- W2080151330 cites W36053226 @default.
- W2080151330 doi "https://doi.org/10.1016/0006-2952(95)98507-6" @default.
- W2080151330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7748191" @default.
- W2080151330 hasPublicationYear "1995" @default.
- W2080151330 type Work @default.
- W2080151330 sameAs 2080151330 @default.
- W2080151330 citedByCount "27" @default.
- W2080151330 countsByYear W20801513302012 @default.
- W2080151330 countsByYear W20801513302016 @default.
- W2080151330 countsByYear W20801513302018 @default.
- W2080151330 crossrefType "journal-article" @default.
- W2080151330 hasAuthorship W2080151330A5007758316 @default.
- W2080151330 hasAuthorship W2080151330A5043268325 @default.
- W2080151330 hasAuthorship W2080151330A5044130061 @default.
- W2080151330 hasAuthorship W2080151330A5085298324 @default.
- W2080151330 hasConcept C162356407 @default.
- W2080151330 hasConcept C179998833 @default.
- W2080151330 hasConcept C185592680 @default.
- W2080151330 hasConcept C192989942 @default.
- W2080151330 hasConcept C2776940978 @default.
- W2080151330 hasConcept C2777477808 @default.
- W2080151330 hasConcept C31827203 @default.
- W2080151330 hasConcept C40684141 @default.
- W2080151330 hasConcept C43617362 @default.
- W2080151330 hasConcept C55493867 @default.
- W2080151330 hasConcept C62231903 @default.
- W2080151330 hasConcept C64489805 @default.
- W2080151330 hasConceptScore W2080151330C162356407 @default.
- W2080151330 hasConceptScore W2080151330C179998833 @default.
- W2080151330 hasConceptScore W2080151330C185592680 @default.
- W2080151330 hasConceptScore W2080151330C192989942 @default.
- W2080151330 hasConceptScore W2080151330C2776940978 @default.
- W2080151330 hasConceptScore W2080151330C2777477808 @default.
- W2080151330 hasConceptScore W2080151330C31827203 @default.
- W2080151330 hasConceptScore W2080151330C40684141 @default.
- W2080151330 hasConceptScore W2080151330C43617362 @default.
- W2080151330 hasConceptScore W2080151330C55493867 @default.
- W2080151330 hasConceptScore W2080151330C62231903 @default.
- W2080151330 hasConceptScore W2080151330C64489805 @default.
- W2080151330 hasIssue "8" @default.
- W2080151330 hasLocation W20801513301 @default.
- W2080151330 hasLocation W20801513302 @default.
- W2080151330 hasOpenAccess W2080151330 @default.
- W2080151330 hasPrimaryLocation W20801513301 @default.
- W2080151330 hasRelatedWork W1277187992 @default.
- W2080151330 hasRelatedWork W1863131412 @default.
- W2080151330 hasRelatedWork W1965571376 @default.
- W2080151330 hasRelatedWork W1969034512 @default.
- W2080151330 hasRelatedWork W2003174742 @default.
- W2080151330 hasRelatedWork W2014605936 @default.
- W2080151330 hasRelatedWork W2027593566 @default.
- W2080151330 hasRelatedWork W2048027784 @default.
- W2080151330 hasRelatedWork W2592318430 @default.
- W2080151330 hasRelatedWork W2787820172 @default.
- W2080151330 hasVolume "49" @default.
- W2080151330 isParatext "false" @default.
- W2080151330 isRetracted "false" @default.
- W2080151330 magId "2080151330" @default.
- W2080151330 workType "article" @default.