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- W2080201690 abstract "Abstract A method was described for the fast and direct determination of small inorganic anions in samples containing large amounts of proteins, such as blood serum or milk. The method was based on gravity injection in a low separation voltage (5 kV)—short tube (Ld = 11 cm) capillary zone electrophoresis (CZE) system for the fast and quantitative determination of chloride, sulfate, phosphate, bicarbonate in serum and milk samples. Baseline separation of inorganic and organic anions was achieved within 50 s. Indirect absorbance detection of anions was accomplished with a chromate-based background electrolyte, modified with cetyltrimethylammonium bromide (CTAB) and acetonitrile at pH 9.3, prevented protein adsorption on the capillary inner walls. Therefore, no sample pretreatment, except for dilution, was required. The effect of the pH, the concentration of electrolyte and modifier on the resolution was investigated. The relative standard deviation (RSD) for the analysis of anions was less than 1–1.8% for the migration time and 1–6.4% for the peak area. The limit of detection was from 0.3 to 1.0 mg/L for the seven anions. Standard addition recoveries of anions in serum and milk samples were between 91 and 104%. This method has been shown promising results for the determination of chloride, sulfate, phosphate, bicarbonate in serum and milk samples." @default.
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- W2080201690 date "2003-01-04" @default.
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- W2080201690 title "Fast and Direct Determination of Small Anions in Proteinaceous Samples Using Low Voltage-Short Tube Capillary Zone Electrophoresis System" @default.
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- W2080201690 doi "https://doi.org/10.1081/al-120017703" @default.
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