Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080203602> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2080203602 endingPage "198" @default.
- W2080203602 startingPage "191" @default.
- W2080203602 abstract "To improve the biocompatibility of a gene vector and to avoid its being eliminated by the immune system, polyethylenimine (PEI) was modified with poly(ethylene glycol) (PEG) before G250 monoclonal antibody (mAb) conjugation. G250-PEI-PEG was capable of forming complexes with DNA in the right size distribution, and the G250 mAb modification significantly improved PEI transfection of G250-positive cells. The highest transfection efficiency was seen in HeLa cells as determined by flow cytometry after transfection with the gene encoding green fluorescent protein: 2-fold higher compared with the transfection of HepG2 cells. Blocking the surface antigen on the cell membrane of HeLa cells by incubation with free G250 mAb, or by downregulating G250 expression by small interfering RNA transfection, resulted in a remarkable decrease in transfection efficiency. These data indicate the targeting effect of G250 antibody modification. The presence of serum decreased transfection efficiency in a concentration-dependent manner. However, the transfection of HeLa cells with G250-PEI-PEG remained significant in the presence of 30% serum. In an in vivo study, G250-PEI-PEG exhibited high transfection efficiency in tumors. In addition, pathological analysis did not show obvious toxicity caused by the materials used. These suggest that PEG- and G250 mAb-modified PEI could be a useful nonviral gene vector for in vivo study." @default.
- W2080203602 created "2016-06-24" @default.
- W2080203602 creator A5010656036 @default.
- W2080203602 creator A5024268530 @default.
- W2080203602 creator A5028875731 @default.
- W2080203602 creator A5044243840 @default.
- W2080203602 creator A5056036756 @default.
- W2080203602 creator A5058855220 @default.
- W2080203602 date "2010-02-01" @default.
- W2080203602 modified "2023-10-17" @default.
- W2080203602 title "Poly(ethylene glycol)-Grafted Polyethylenimine Modified with G250 Monoclonal Antibody for Tumor Gene Therapy" @default.
- W2080203602 cites W1483734912 @default.
- W2080203602 cites W1970708694 @default.
- W2080203602 cites W1973458116 @default.
- W2080203602 cites W1973826816 @default.
- W2080203602 cites W1992998779 @default.
- W2080203602 cites W2002472186 @default.
- W2080203602 cites W2010655741 @default.
- W2080203602 cites W2019026359 @default.
- W2080203602 cites W2029914238 @default.
- W2080203602 cites W2048928172 @default.
- W2080203602 cites W2050858497 @default.
- W2080203602 cites W2050932091 @default.
- W2080203602 cites W2076941870 @default.
- W2080203602 cites W2083597639 @default.
- W2080203602 cites W2137000696 @default.
- W2080203602 cites W2154329741 @default.
- W2080203602 cites W2330673049 @default.
- W2080203602 cites W353012429 @default.
- W2080203602 doi "https://doi.org/10.1089/hum.2009.010" @default.
- W2080203602 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19788387" @default.
- W2080203602 hasPublicationYear "2010" @default.
- W2080203602 type Work @default.
- W2080203602 sameAs 2080203602 @default.
- W2080203602 citedByCount "21" @default.
- W2080203602 countsByYear W20802036022012 @default.
- W2080203602 countsByYear W20802036022013 @default.
- W2080203602 countsByYear W20802036022014 @default.
- W2080203602 countsByYear W20802036022015 @default.
- W2080203602 countsByYear W20802036022017 @default.
- W2080203602 countsByYear W20802036022019 @default.
- W2080203602 countsByYear W20802036022021 @default.
- W2080203602 crossrefType "journal-article" @default.
- W2080203602 hasAuthorship W2080203602A5010656036 @default.
- W2080203602 hasAuthorship W2080203602A5024268530 @default.
- W2080203602 hasAuthorship W2080203602A5028875731 @default.
- W2080203602 hasAuthorship W2080203602A5044243840 @default.
- W2080203602 hasAuthorship W2080203602A5056036756 @default.
- W2080203602 hasAuthorship W2080203602A5058855220 @default.
- W2080203602 hasConcept C104317684 @default.
- W2080203602 hasConcept C111599444 @default.
- W2080203602 hasConcept C135983454 @default.
- W2080203602 hasConcept C153911025 @default.
- W2080203602 hasConcept C159654299 @default.
- W2080203602 hasConcept C185592680 @default.
- W2080203602 hasConcept C202751555 @default.
- W2080203602 hasConcept C203014093 @default.
- W2080203602 hasConcept C2775925850 @default.
- W2080203602 hasConcept C2777366897 @default.
- W2080203602 hasConcept C54009773 @default.
- W2080203602 hasConcept C542903549 @default.
- W2080203602 hasConcept C55493867 @default.
- W2080203602 hasConcept C86803240 @default.
- W2080203602 hasConceptScore W2080203602C104317684 @default.
- W2080203602 hasConceptScore W2080203602C111599444 @default.
- W2080203602 hasConceptScore W2080203602C135983454 @default.
- W2080203602 hasConceptScore W2080203602C153911025 @default.
- W2080203602 hasConceptScore W2080203602C159654299 @default.
- W2080203602 hasConceptScore W2080203602C185592680 @default.
- W2080203602 hasConceptScore W2080203602C202751555 @default.
- W2080203602 hasConceptScore W2080203602C203014093 @default.
- W2080203602 hasConceptScore W2080203602C2775925850 @default.
- W2080203602 hasConceptScore W2080203602C2777366897 @default.
- W2080203602 hasConceptScore W2080203602C54009773 @default.
- W2080203602 hasConceptScore W2080203602C542903549 @default.
- W2080203602 hasConceptScore W2080203602C55493867 @default.
- W2080203602 hasConceptScore W2080203602C86803240 @default.
- W2080203602 hasIssue "2" @default.
- W2080203602 hasLocation W20802036021 @default.
- W2080203602 hasLocation W20802036022 @default.
- W2080203602 hasOpenAccess W2080203602 @default.
- W2080203602 hasPrimaryLocation W20802036021 @default.
- W2080203602 hasRelatedWork W2009444013 @default.
- W2080203602 hasRelatedWork W2052288565 @default.
- W2080203602 hasRelatedWork W2094101506 @default.
- W2080203602 hasRelatedWork W2352254145 @default.
- W2080203602 hasRelatedWork W2362737558 @default.
- W2080203602 hasRelatedWork W2424993904 @default.
- W2080203602 hasRelatedWork W2586189433 @default.
- W2080203602 hasRelatedWork W2909823711 @default.
- W2080203602 hasRelatedWork W2914720861 @default.
- W2080203602 hasRelatedWork W3173318358 @default.
- W2080203602 hasVolume "21" @default.
- W2080203602 isParatext "false" @default.
- W2080203602 isRetracted "false" @default.
- W2080203602 magId "2080203602" @default.
- W2080203602 workType "article" @default.