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- W2080208258 abstract "The purpose of this study is to investigate the structural features of corneal edema by multiphoton fluorescence and second harmonic generation (SHG) microscopy and the potential of this technique as a clinical, in vivo monitoring technique for cornea disease diagnosis. Corneal edema is a build up of fluid or swelling of the cornea that can produce clouding of the cornea and significant decrease in vision. Since collagen can be induced to generate strong second harmonic generation (SHG) signal, multiphoton excitation provide direct visualization of collagen orientation within corneal stroma.In this study, normal and over-hydrated bovine corneal specimens used in this study were observed to distinguish the structural alteration due to edema. We collected forward and backward SHG signals simultaneously within different depths and also with large region images. From the result, we found that the organization of corneal stroma was not significantly altered, except for the increase of interfibrillar spacing. This structural information provided by multiphoton imaging may help the evaluation of the necessity for full-thickness corneal transplantation in pathological cases." @default.
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- W2080208258 date "2009-02-01" @default.
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- W2080208258 title "Second Harmonic Generation Microscopy Characterization of Corneal Edema" @default.
- W2080208258 doi "https://doi.org/10.1016/j.bpj.2008.12.1465" @default.
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