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• W2080215172 abstract "We report the first analysis of the soluble sub-proteome of the obligate thermophile, Geobacillus thermoleovorans T80, utilizing a robust multidimensional protein identification protocol. A total of 1,336 proteins were initially identified utilizing automated MS/MS identification software. Intensive manual curation resulted in a final list containing a total of 294 unique proteins. Physiochemical characterization and functional classification of the soluble sub-proteome was carried out. The strategy has allowed us to gain an insight into the cellular processes of this obligate thermophile, identifying a variety of proteins known to play a role in stress response. Included within these were a number of sigma factors such as sigma(A) that initiate transcription of the heat shock operons controlled by the HrcA-CIRCE complex within gram positive bacteria. In addition, it has enabled us to assign a degree of functionality to 29 out of 36 gene products detected in this study that were hitherto described as being only hypothetical conserved proteins." @default.
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• W2080215172 date "2006-02-24" @default.
• W2080215172 modified "2023-10-14" @default.
• W2080215172 title "Top-Down Proteomic Analysis of the Soluble Sub-Proteome of the Obligate Thermophile, <i>Geobacillus </i><i>t</i><i>hermoleovorans</i> T80: Insights into Its Cellular Processes" @default.
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• W2080215172 doi "https://doi.org/10.1021/pr0504642" @default.
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