Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080228510> ?p ?o ?g. }
- W2080228510 endingPage "1643" @default.
- W2080228510 startingPage "1630" @default.
- W2080228510 abstract "Sec9p and Spo20p are two SNAP25 family SNARE proteins specialized for different developmental stages in yeast. Sec9p interacts with Sso1/2p and Snc1/2p to mediate intracellular trafficking between post‐Golgi vesicles and the plasma membrane during vegetative growth. Spo20p replaces Sec9p in the generation of prospore membranes during sporulation. The function of Spo20p requires enzymatically active Spo14p, which is a phosphatidylcholine (PC)‐specific phospholipase D that hydrolyzes PC to generate phosphatidic acid (PA). Phosphatidic acid is required to localize Spo20p properly during sporulation; however, it seems to have additional roles that are not fully understood. Here we compared the fusion mediated by all combinations of the Sec9p or Spo20p C‐terminal domains with Sso1p/Sso2p and Snc1p/Snc2p. Our results show that Spo20p forms a less efficient SNARE complex than Sec9p. The combination of Sso2p/Spo20c is the least fusogenic t‐SNARE complex. Incorporation of PA in the lipid bilayer stimulates SNARE‐mediated membrane fusion by all t‐SNARE complexes, likely by decreasing the energetic barrier during membrane merger. This effect may allow the weak SNARE complex containing Spo20p to function during sporulation. In addition, PA can directly interact with the juxtamembrane region of Sso1p, which contributes to the stimulatory effects of PA on membrane fusion. Our results suggest that the fusion strength of SNAREs, the composition of organelle lipids and lipid–SNARE interactions may be coordinately regulated to control the rate and specificity of membrane fusion." @default.
- W2080228510 created "2016-06-24" @default.
- W2080228510 creator A5014295844 @default.
- W2080228510 creator A5034238450 @default.
- W2080228510 creator A5049515262 @default.
- W2080228510 creator A5063343005 @default.
- W2080228510 creator A5065008527 @default.
- W2080228510 date "2007-07-22" @default.
- W2080228510 modified "2023-10-18" @default.
- W2080228510 title "In Vitro Fusion Catalyzed by the Sporulation-Specific t-SNARE Light-Chain Spo20p is Stimulated by Phosphatidic Acid" @default.
- W2080228510 cites W1485562018 @default.
- W2080228510 cites W1540559701 @default.
- W2080228510 cites W1570678799 @default.
- W2080228510 cites W1597709568 @default.
- W2080228510 cites W1822252127 @default.
- W2080228510 cites W1891504318 @default.
- W2080228510 cites W1905416680 @default.
- W2080228510 cites W1927096570 @default.
- W2080228510 cites W1939706863 @default.
- W2080228510 cites W1966600110 @default.
- W2080228510 cites W197316475 @default.
- W2080228510 cites W1974054235 @default.
- W2080228510 cites W1976283234 @default.
- W2080228510 cites W1976334985 @default.
- W2080228510 cites W1977017692 @default.
- W2080228510 cites W1977574098 @default.
- W2080228510 cites W1982501876 @default.
- W2080228510 cites W1985321331 @default.
- W2080228510 cites W1998062844 @default.
- W2080228510 cites W2001389730 @default.
- W2080228510 cites W2002669633 @default.
- W2080228510 cites W2009417622 @default.
- W2080228510 cites W2016235845 @default.
- W2080228510 cites W2020222865 @default.
- W2080228510 cites W2022977995 @default.
- W2080228510 cites W2030972230 @default.
- W2080228510 cites W2031212561 @default.
- W2080228510 cites W2037773086 @default.
- W2080228510 cites W2038263678 @default.
- W2080228510 cites W2040169742 @default.
- W2080228510 cites W2045198784 @default.
- W2080228510 cites W2048062655 @default.
- W2080228510 cites W2049199355 @default.
- W2080228510 cites W2051103934 @default.
- W2080228510 cites W2056182641 @default.
- W2080228510 cites W2056293200 @default.
- W2080228510 cites W2059775037 @default.
- W2080228510 cites W2063253418 @default.
- W2080228510 cites W2063851345 @default.
- W2080228510 cites W2075240236 @default.
- W2080228510 cites W2077451533 @default.
- W2080228510 cites W2081151685 @default.
- W2080228510 cites W2082497364 @default.
- W2080228510 cites W2083258595 @default.
- W2080228510 cites W2085680244 @default.
- W2080228510 cites W2089333920 @default.
- W2080228510 cites W2091545748 @default.
- W2080228510 cites W2094479838 @default.
- W2080228510 cites W2096005517 @default.
- W2080228510 cites W2102019596 @default.
- W2080228510 cites W2105252089 @default.
- W2080228510 cites W2108369327 @default.
- W2080228510 cites W2112488976 @default.
- W2080228510 cites W2116076986 @default.
- W2080228510 cites W2117998295 @default.
- W2080228510 cites W2120126668 @default.
- W2080228510 cites W2120694601 @default.
- W2080228510 cites W2121468553 @default.
- W2080228510 cites W2127671558 @default.
- W2080228510 cites W2150341205 @default.
- W2080228510 cites W2163795610 @default.
- W2080228510 cites W2164433356 @default.
- W2080228510 cites W23204795 @default.
- W2080228510 cites W4211224007 @default.
- W2080228510 doi "https://doi.org/10.1111/j.1600-0854.2007.00628.x" @default.
- W2080228510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17714435" @default.
- W2080228510 hasPublicationYear "2007" @default.
- W2080228510 type Work @default.
- W2080228510 sameAs 2080228510 @default.
- W2080228510 citedByCount "39" @default.
- W2080228510 countsByYear W20802285102012 @default.
- W2080228510 countsByYear W20802285102013 @default.
- W2080228510 countsByYear W20802285102014 @default.
- W2080228510 countsByYear W20802285102015 @default.
- W2080228510 countsByYear W20802285102016 @default.
- W2080228510 countsByYear W20802285102018 @default.
- W2080228510 countsByYear W20802285102019 @default.
- W2080228510 countsByYear W20802285102020 @default.
- W2080228510 countsByYear W20802285102021 @default.
- W2080228510 crossrefType "journal-article" @default.
- W2080228510 hasAuthorship W2080228510A5014295844 @default.
- W2080228510 hasAuthorship W2080228510A5034238450 @default.
- W2080228510 hasAuthorship W2080228510A5049515262 @default.
- W2080228510 hasAuthorship W2080228510A5063343005 @default.
- W2080228510 hasAuthorship W2080228510A5065008527 @default.
- W2080228510 hasConcept C102747710 @default.
- W2080228510 hasConcept C103038307 @default.
- W2080228510 hasConcept C119062480 @default.