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• W2080237747 startingPage "183" @default.
• W2080237747 abstract "Many ex vivo factors influence the yield of recombinant protein produced via AcMNPV (Autographa californica multiple nucleocapsid nuclear polyhedrosis virus) in Trichoplusia ni (T. ni) larvae. Among these are: the method of infection, the time of infection, the virus load, and the time of harvest. In vivo strategies, however, that attempt to manipulate host function in this and other expression systems have largely been ignored. In this work, RNA interference (RNAi) is shown as an effective metabolic engineering controller to downregulate targeted gene expression. Specifically, RNAi was made to virus-encoded gfpuv and was found to inhibit the production of GFPuv in larvae when injected within an 18-h window (before and after) of baculovirus infection. The level of inhibition was found to depend, both in duration and extent, on the concentration of injected RNAi. That relatively low levels of RNAi can inhibit protein synthesis driven by the strong polyhedrin (polh) promoter of AcMNPV, suggests that RNAi will find utility as an in vivo metabolic controller in metabolic engineering studies such as this one pertaining to protein expression." @default.
• W2080237747 created "2016-06-24" @default.
• W2080237747 creator A5027095675 @default.
• W2080237747 creator A5048856563 @default.
• W2080237747 date "2003-07-01" @default.
• W2080237747 modified "2023-09-29" @default.
• W2080237747 title "RNA interference as a metabolic engineering tool: potential for in vivo control of protein expression in an insect larval model" @default.
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• W2080237747 doi "https://doi.org/10.1016/s1096-7176(03)00027-2" @default.
• W2080237747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12948752" @default.
• W2080237747 hasPublicationYear "2003" @default.
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