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- W2080257569 abstract "Abstract We developed a kinetic assay using a monolayer of differentiated respiratory epithelium in culture to assess bacterial adherence. Mean residence time of bacteria in the tissue culture chamber was estimated from a model-independent (moment) analysis of the rate of bacterial washout from perfused Rose chambers. Results with this method compared favorably with visual assessment of adherence and double radiolabel method with H. influenzae . Adherence was assessed with low inoculae of H. influenzae, P. cepacia and P. aeruginosa avoiding cytotoxic effects seen when large inoculae are added to eukaryotic cells. This method will provide a means of assessing adherence of pathogenic respiratory bacteria to their cellular target at low inoculae." @default.
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- W2080257569 date "1989-12-01" @default.
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- W2080257569 title "Kinetic measurement of bacterial adherence to eukaryotic cells" @default.
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- W2080257569 doi "https://doi.org/10.1016/0167-7012(89)90014-6" @default.
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