Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080264210> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2080264210 endingPage "61" @default.
- W2080264210 startingPage "53" @default.
- W2080264210 abstract "PCR assay has been shown to be a promising option for the diagnosis of brucellosis. However, few studies have been performed with field samples in order to evaluate the assay as a diagnostic tool. In this study, routine use of a species-specific PCR assay previously developed for the identification of Brucella cultures was assessed for the detection of Brucella DNA directly from the stomach contents of aborted sheep fetuses. The assay is based on the insertion sequence IS711 in the Brucella chromosome. In the study, during 3 successive lambing seasons (1998-1999, 1999-2000 and 2000-2001) 126 aborted fetus samples each from different flocks and locations were examined. Brucella strains were isolated from 39 (31%) of the samples and all of the strains were identified as Brucella melitensis by biochemical characteristics, agglutination with monospecific A and M sera and PCR. Thirty-seven of 39 B. melitensis isolates were biotyped as biotype 3, and 2 isolates as biotype 1. From 38 of 39 culture positive fetal stomach contents B. melitensis-specific DNA was detected by PCR. PCR was found negative in all of the culture negative samples. Compared with culture, sensitivity and specificity of PCR were determined as 97.4 and 100%, respectively. The results indicate that this PCR procedure has a potential for use in routine diagnosis of sheep brucellosis." @default.
- W2080264210 created "2016-06-24" @default.
- W2080264210 creator A5009932379 @default.
- W2080264210 creator A5056863449 @default.
- W2080264210 creator A5080701207 @default.
- W2080264210 date "2003-05-01" @default.
- W2080264210 modified "2023-09-23" @default.
- W2080264210 title "Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples" @default.
- W2080264210 cites W1492017835 @default.
- W2080264210 cites W1523831561 @default.
- W2080264210 cites W1528489647 @default.
- W2080264210 cites W1593542282 @default.
- W2080264210 cites W1955241704 @default.
- W2080264210 cites W1964519551 @default.
- W2080264210 cites W2033113600 @default.
- W2080264210 cites W2040823232 @default.
- W2080264210 cites W2061788711 @default.
- W2080264210 cites W2067543094 @default.
- W2080264210 cites W2085293251 @default.
- W2080264210 cites W2092776911 @default.
- W2080264210 cites W2134645425 @default.
- W2080264210 cites W2163568277 @default.
- W2080264210 cites W2166671413 @default.
- W2080264210 cites W2171050326 @default.
- W2080264210 doi "https://doi.org/10.1016/s0378-1135(02)00442-x" @default.
- W2080264210 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12591206" @default.
- W2080264210 hasPublicationYear "2003" @default.
- W2080264210 type Work @default.
- W2080264210 sameAs 2080264210 @default.
- W2080264210 citedByCount "85" @default.
- W2080264210 countsByYear W20802642102012 @default.
- W2080264210 countsByYear W20802642102013 @default.
- W2080264210 countsByYear W20802642102014 @default.
- W2080264210 countsByYear W20802642102015 @default.
- W2080264210 countsByYear W20802642102016 @default.
- W2080264210 countsByYear W20802642102017 @default.
- W2080264210 countsByYear W20802642102018 @default.
- W2080264210 countsByYear W20802642102019 @default.
- W2080264210 countsByYear W20802642102020 @default.
- W2080264210 countsByYear W20802642102021 @default.
- W2080264210 countsByYear W20802642102022 @default.
- W2080264210 countsByYear W20802642102023 @default.
- W2080264210 crossrefType "journal-article" @default.
- W2080264210 hasAuthorship W2080264210A5009932379 @default.
- W2080264210 hasAuthorship W2080264210A5056863449 @default.
- W2080264210 hasAuthorship W2080264210A5080701207 @default.
- W2080264210 hasConcept C104317684 @default.
- W2080264210 hasConcept C134215735 @default.
- W2080264210 hasConcept C151730666 @default.
- W2080264210 hasConcept C159047783 @default.
- W2080264210 hasConcept C172680121 @default.
- W2080264210 hasConcept C2777692091 @default.
- W2080264210 hasConcept C2779234561 @default.
- W2080264210 hasConcept C2780767784 @default.
- W2080264210 hasConcept C2781028839 @default.
- W2080264210 hasConcept C2781429187 @default.
- W2080264210 hasConcept C2909361669 @default.
- W2080264210 hasConcept C49105822 @default.
- W2080264210 hasConcept C54355233 @default.
- W2080264210 hasConcept C86803240 @default.
- W2080264210 hasConcept C89423630 @default.
- W2080264210 hasConcept C90583042 @default.
- W2080264210 hasConceptScore W2080264210C104317684 @default.
- W2080264210 hasConceptScore W2080264210C134215735 @default.
- W2080264210 hasConceptScore W2080264210C151730666 @default.
- W2080264210 hasConceptScore W2080264210C159047783 @default.
- W2080264210 hasConceptScore W2080264210C172680121 @default.
- W2080264210 hasConceptScore W2080264210C2777692091 @default.
- W2080264210 hasConceptScore W2080264210C2779234561 @default.
- W2080264210 hasConceptScore W2080264210C2780767784 @default.
- W2080264210 hasConceptScore W2080264210C2781028839 @default.
- W2080264210 hasConceptScore W2080264210C2781429187 @default.
- W2080264210 hasConceptScore W2080264210C2909361669 @default.
- W2080264210 hasConceptScore W2080264210C49105822 @default.
- W2080264210 hasConceptScore W2080264210C54355233 @default.
- W2080264210 hasConceptScore W2080264210C86803240 @default.
- W2080264210 hasConceptScore W2080264210C89423630 @default.
- W2080264210 hasConceptScore W2080264210C90583042 @default.
- W2080264210 hasIssue "1" @default.
- W2080264210 hasLocation W20802642101 @default.
- W2080264210 hasLocation W20802642102 @default.
- W2080264210 hasOpenAccess W2080264210 @default.
- W2080264210 hasPrimaryLocation W20802642101 @default.
- W2080264210 hasRelatedWork W1957726808 @default.
- W2080264210 hasRelatedWork W2012976806 @default.
- W2080264210 hasRelatedWork W2040928049 @default.
- W2080264210 hasRelatedWork W2080264210 @default.
- W2080264210 hasRelatedWork W2081338764 @default.
- W2080264210 hasRelatedWork W2141560699 @default.
- W2080264210 hasRelatedWork W2253648943 @default.
- W2080264210 hasRelatedWork W2384078366 @default.
- W2080264210 hasRelatedWork W3029757537 @default.
- W2080264210 hasRelatedWork W3087998175 @default.
- W2080264210 hasVolume "93" @default.
- W2080264210 isParatext "false" @default.
- W2080264210 isRetracted "false" @default.
- W2080264210 magId "2080264210" @default.
- W2080264210 workType "article" @default.