FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080271937> ?p ?o ?g. }

• W2080271937 endingPage "61" @default.
• W2080271937 startingPage "55" @default.
• W2080271937 abstract "The enzymatic synthesis of 7-deazapurine nucleoside containing DNA (501 bp) is performed by PCR-amplification (Taq polymerase) using a pUC18 plasmid DNA as template and the triphosphates of 7-deaza-2'-deoxyguanosine (c7Gd), -adenosine (c7Ad) and -inosine (c7Id). c7GdTP can fully replace dGTP resulting in a completely modified DNA-fragment of defined size and sequence. The other two 7-deazapurine triphosphates (c7AdTP) and (c7IdTP) require the presence of the parent purine 2'-deoxyribonucleotides. In purine/7-deazapurine nucleotide mixtures Taq polymerase prefers purine over 7-deazapurine nucleotides but accepts c7GdTP much better than c7AdTP or c7IdTP. As incorporation of 7-deazapurine nucleotides represents a modification of the major groove of DNA it can be used to probe DNA/protein interaction. Regioselective phosphodiester hydrolysis of the modified DNA-fragments was studied with 28 endodeoxyribonucleases. c7Gd is able to protect the DNA from the phosphodiester hydrolysis in more than 20 cases, only a few enzymes (Mae III, Rsa I, Hind III, Pvu II or Taq I) do still hydrolyze the modified DNA. c7Ad protects DNA less efficiently, as this DNA could only be modified in part. The absence of N-7 as potential binding position or a geometric distortion of the recognition duplex caused by the 7-deazapurine base can account for protection of hydrolysis." @default.
• W2080271937 created "2016-06-24" @default.
• W2080271937 creator A5011294725 @default.
• W2080271937 creator A5060869360 @default.
• W2080271937 date "1992-01-01" @default.
• W2080271937 modified "2023-10-13" @default.
• W2080271937 title "7-Deazapurine containing DNA: efficiency of c⁷G_dTP, c⁷A_dTP and c⁷l_dTP incorporation during PCR amplification and protection from endodeoxyribonuclease hydrolysis" @default.
• W2080271937 cites W1481640863 @default.
• W2080271937 cites W1517480469 @default.
• W2080271937 cites W1629931862 @default.
• W2080271937 cites W1982878977 @default.
• W2080271937 cites W2013408101 @default.
• W2080271937 cites W2016260960 @default.
• W2080271937 cites W2022719676 @default.
• W2080271937 cites W2027805425 @default.
• W2080271937 cites W2028622989 @default.
• W2080271937 cites W2031500868 @default.
• W2080271937 cites W2031928161 @default.
• W2080271937 cites W2032118018 @default.
• W2080271937 cites W2050879305 @default.
• W2080271937 cites W2053099555 @default.
• W2080271937 cites W2054919238 @default.
• W2080271937 cites W2057556769 @default.
• W2080271937 cites W2061057528 @default.
• W2080271937 cites W2079672141 @default.
• W2080271937 cites W2089811070 @default.
• W2080271937 cites W2100067555 @default.
• W2080271937 cites W2112714750 @default.
• W2080271937 cites W2133042489 @default.
• W2080271937 cites W2138270253 @default.
• W2080271937 cites W2151465840 @default.
• W2080271937 cites W281526338 @default.
• W2080271937 doi "https://doi.org/10.1093/nar/20.1.55" @default.
• W2080271937 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/310325" @default.
• W2080271937 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1738604" @default.
• W2080271937 hasPublicationYear "1992" @default.
• W2080271937 type Work @default.
• W2080271937 sameAs 2080271937 @default.
• W2080271937 citedByCount "64" @default.
• W2080271937 countsByYear W20802719372012 @default.
• W2080271937 countsByYear W20802719372013 @default.
• W2080271937 countsByYear W20802719372014 @default.
• W2080271937 countsByYear W20802719372015 @default.
• W2080271937 countsByYear W20802719372016 @default.
• W2080271937 countsByYear W20802719372017 @default.
• W2080271937 countsByYear W20802719372018 @default.
• W2080271937 countsByYear W20802719372020 @default.
• W2080271937 countsByYear W20802719372021 @default.
• W2080271937 countsByYear W20802719372023 @default.
• W2080271937 crossrefType "journal-article" @default.
• W2080271937 hasAuthorship W2080271937A5011294725 @default.
• W2080271937 hasAuthorship W2080271937A5060869360 @default.
• W2080271937 hasBestOaLocation W20802719372 @default.
• W2080271937 hasConcept C104317684 @default.
• W2080271937 hasConcept C111921641 @default.
• W2080271937 hasConcept C129312508 @default.
• W2080271937 hasConcept C153911025 @default.
• W2080271937 hasConcept C181199279 @default.
• W2080271937 hasConcept C185581394 @default.
• W2080271937 hasConcept C185592680 @default.
• W2080271937 hasConcept C2756471 @default.
• W2080271937 hasConcept C2776476023 @default.
• W2080271937 hasConcept C2776543447 @default.
• W2080271937 hasConcept C38441620 @default.
• W2080271937 hasConcept C512185932 @default.
• W2080271937 hasConcept C552990157 @default.
• W2080271937 hasConcept C55493867 @default.
• W2080271937 hasConcept C67705224 @default.
• W2080271937 hasConcept C71240020 @default.
• W2080271937 hasConcept C86803240 @default.
• W2080271937 hasConceptScore W2080271937C104317684 @default.
• W2080271937 hasConceptScore W2080271937C111921641 @default.
• W2080271937 hasConceptScore W2080271937C129312508 @default.
• W2080271937 hasConceptScore W2080271937C153911025 @default.
• W2080271937 hasConceptScore W2080271937C181199279 @default.
• W2080271937 hasConceptScore W2080271937C185581394 @default.
• W2080271937 hasConceptScore W2080271937C185592680 @default.
• W2080271937 hasConceptScore W2080271937C2756471 @default.
• W2080271937 hasConceptScore W2080271937C2776476023 @default.
• W2080271937 hasConceptScore W2080271937C2776543447 @default.
• W2080271937 hasConceptScore W2080271937C38441620 @default.
• W2080271937 hasConceptScore W2080271937C512185932 @default.
• W2080271937 hasConceptScore W2080271937C552990157 @default.
• W2080271937 hasConceptScore W2080271937C55493867 @default.
• W2080271937 hasConceptScore W2080271937C67705224 @default.
• W2080271937 hasConceptScore W2080271937C71240020 @default.
• W2080271937 hasConceptScore W2080271937C86803240 @default.
• W2080271937 hasIssue "1" @default.
• W2080271937 hasLocation W20802719371 @default.
• W2080271937 hasLocation W20802719372 @default.
• W2080271937 hasLocation W20802719373 @default.
• W2080271937 hasOpenAccess W2080271937 @default.
• W2080271937 hasPrimaryLocation W20802719371 @default.
• W2080271937 hasRelatedWork W1991178337 @default.
• W2080271937 hasRelatedWork W2006496544 @default.
• W2080271937 hasRelatedWork W2014241579 @default.
• W2080271937 hasRelatedWork W2051131639 @default.
• W2080271937 hasRelatedWork W2066526988 @default.

• next