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• W2080285220 abstract "Wortmannin, a specific inhibitor of myosin light chain kinase (MLCK), enhanced carbachol-induced formation of [3H]phosphatidyl-ethanol ([3H]PEt), a marker of phospholipase D (PLD)activity, in [3H]palmitic acid-labeled PC12 cells. The apparent EC50 value was 1.5 μM, and the effect was maximal at 3 μM and slightly attenuated at higher concentration. Wortmannin alone had no significant effect on [3H]PEt formation. The enhancing effect of wortmannin was observed at the initial increasing phase of [3H]PEt formation but not at the subsequent plateau phase. Wortmannin enhanced also phorbol ester-induced PLD activation. Although the precise mechanism remains to be clarified, these results suggest that MLCK may be involved in PLD regulation in PC 12 cells." @default.
• W2080285220 created "2016-06-24" @default.
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• W2080285220 date "1992-10-01" @default.
• W2080285220 modified "2023-10-02" @default.
• W2080285220 title "Enhancing effect of wortmannin on muscarinic stimulation of phospholipase d in rat pheochromocytoma PC12 cells" @default.
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• W2080285220 doi "https://doi.org/10.1016/0006-291x(92)91085-5" @default.
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