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• W2080302571 abstract "Thrombin initiates many physiological processes in platelets and other megakaryocyte-lineage cells by interacting with surface receptors and generating rises in cytoplasmic Ca2+; these rises result from both Ca2+ release from intracellular stores and receptor-mediated Ca2+ entry. Regulators that limit Ca2+ entry after its initiation by thrombin have not been identified. In this study, prevention of expression of a single protein kinase C isoenzyme (PKCβ) by antisense cDNA overexpressed in HEL cells, a human megakaryoblastic cell line that expresses thrombin receptors, promotes thrombin receptor-mediated Ca2+ entry without altering thrombin-induced intracellular release of Ca2+. The cytoplasmic Ca2+ rise initiated by endoperoxide analogs was not affected by inhibiting PKCβ. Overexpression of a cDNA encoding wild-type PKCβ mutated to prevent recognition by the antisense cDNA abolished the enhancement of Ca2+ influx following thrombin. Thus, PKCβ appears to be a specific negative regulator of thrombin receptor-mediated Ca2+ entry. Thrombin initiates many physiological processes in platelets and other megakaryocyte-lineage cells by interacting with surface receptors and generating rises in cytoplasmic Ca2+; these rises result from both Ca2+ release from intracellular stores and receptor-mediated Ca2+ entry. Regulators that limit Ca2+ entry after its initiation by thrombin have not been identified. In this study, prevention of expression of a single protein kinase C isoenzyme (PKCβ) by antisense cDNA overexpressed in HEL cells, a human megakaryoblastic cell line that expresses thrombin receptors, promotes thrombin receptor-mediated Ca2+ entry without altering thrombin-induced intracellular release of Ca2+. The cytoplasmic Ca2+ rise initiated by endoperoxide analogs was not affected by inhibiting PKCβ. Overexpression of a cDNA encoding wild-type PKCβ mutated to prevent recognition by the antisense cDNA abolished the enhancement of Ca2+ influx following thrombin. Thus, PKCβ appears to be a specific negative regulator of thrombin receptor-mediated Ca2+ entry." @default.
• W2080302571 created "2016-06-24" @default.
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• W2080302571 date "1995-10-01" @default.
• W2080302571 modified "2023-09-30" @default.
• W2080302571 title "Selective Inhibition of Thrombin Receptor-mediated Ca2+ Entry by Protein Kinase C β" @default.
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• W2080302571 doi "https://doi.org/10.1074/jbc.270.41.23887" @default.
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