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- W2080331212 abstract "Abstract The surfaces of plastic (polystyrene) Petri dishes from several suppliers were discovered to have the useful property of immobilizing cells of the ciliate Tetrahymena thermophilia upon contact in nutrient-free buffer (10 mM Tris, pH 7.4). The procedure works with cells in both logarithmic and stationary growth phase, so long as they are first transferred to nutrient-free buffer, and then added to dishes already containing buffer to a depth of 2–10 mm. Dish surfaces specially treated for tissue cultures are unsuitable for this purpose. Cells can be released from the dish surfaces by the simple addition of growth medium (1% proteose peptone). Immobilized cells are fully competent to complete conjugation or cell division. The technique offers promise for facilitating experiments requiring microinjection, microsurgery, or simply detailed observation of living protozoan cells." @default.
- W2080331212 created "2016-06-24" @default.
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- W2080331212 date "1981-08-01" @default.
- W2080331212 modified "2023-10-14" @default.
- W2080331212 title "A method for the immobilization of living Tetrahymena" @default.
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- W2080331212 doi "https://doi.org/10.1016/0014-4827(81)90431-6" @default.
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