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- W2080353092 abstract "Abstract Enzymatic acetylcholine synthesis by mouse brain, rat sperm, and purified pigeon breast muscle carnitine acetyltransferase was monitored. Optimal assay procedure, choline substrate requirements, the extent of acetylcholine synthesis, and the effects of acetyltransferase inhibitors were investigated to determine if acetylcholine in rat sperm is synthesized by choline acetyltransferase or by another enzyme that utilizes choline as a substrate. Of two assay procedures tested, the liquid cation-exchange procedure utilizing a butyronitrile-tetraphenylboron extraction was judged superior to an anion-exchange resin procedure. The latter procedure gave falsely high acetylcholine synthesis readings due to another acetylated contaminant (probably acetylcarnitine). The K m for choline substrate in acetylcholine syntheses by mouse brain, which is a source of choline acetyltransferase, was 0.623 mM choline; this was 300 times less than the choline substrate K m with rat sperm (207 mM choline) and 80 times less than the K m with purified carnitine acetyltransferase (50.6 mM choline). Rat sperm had a V max [3718 pmoles AcCh · min −1 · (mg protein) −1 ] that was > 2-fold that of mouse brain [1603 pmoles AcCh · min −1 ·(mg protein) −1 ]. A specific inhibitor of choline acetyltransferase, 4-(1-naphthylvinyl)pyridine (500 μM), abolished acetylcholine synthesis by mouse brain, but it caused only a 52 per cent inhibition of acetylcholine synthesis by rat sperm and only a 12 per cent inhibition of acetylcholine synthesis by purified carnitine acetyltransferase. These data indicate that an enzyme other than classical or “true” choline acetyltransferase is responsible for acetylcholine synthesis by rat sperm. Because of the high content of carnitine acetyltransferase in rat sperm and the ability of carnitine acetyltransferase from pigeon breast muscle to synthesize acetylcholine, carnitine acetyltransferase is the most probable enzyme responsible for acetylcholine synthesis in rat sperm." @default.
- W2080353092 created "2016-06-24" @default.
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- W2080353092 date "1981-06-01" @default.
- W2080353092 modified "2023-09-25" @default.
- W2080353092 title "Characterization of enzymatic acetylcholine synthesis by mouse brain, rat sperm, and purified carnitine acetyltransferase" @default.
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- W2080353092 doi "https://doi.org/10.1016/0006-2952(81)90376-2" @default.
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