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- W2080353507 abstract "The embryology of Herminium monorchis (Orchidaceae) was studied using confocal scanning laser microscopy (CSLM), a new technique for embryological studies. This technique may contribute new information to plant embryology. Herminium monorchis has a monosporic embryo sac development. The mature embryo sac is 8-nucleate. Two integuments, both 2-layered, are formed, but only the inner takes part in formation of the micropyle. Double fertilization takes place. The primary endosperm nucleus does not divide, but remains alive at least at the 3-celled stage of embryo development. The three antipodals do not show any sign of degeneration at this stage. THE GENUS Herminium Guett. of the Orchidaceae comprises 30 species. Herminium monorchis (L.) R. Br. is the only species occurring in Europe. The main distribution of the genus is temperate Eurasia, Thailand, the Philippine Islands, and Java (Willis, 1973). Treub (1879) described the embryos of several orchids with their remarkable suspensor haustoria. Among other orchids studied by Treub the late stages in embryo development of H. monorchis were investigated. Mohana Rao and Sood (1979) described the embryo development of H. angustifolium. Investigations of the male and female gametophytes of the same species were made by Sood and Mohana Rao (1986). The aim of the present study is to give a further contribution to the knowledge of the embryology in Herminium. In my embryological studies of the Orchidaceae I use a confocal scanning laser microscope (Fredrikson, Carlsson, and Franksson, 1988). The unique optical sectioning technique makes it possible to study unstained ovules individually. The equilibrium orientation ofthe ovules is such that perfect longitudinal sections can usually be obtained. The sections are only 1 ,um thick and undistorted, and inherently in perfect alignment. The three-dimensional structure of the embryo sac can easily be studied. The sections are photographed from a TV screen." @default.
- W2080353507 created "2016-06-24" @default.
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- W2080353507 date "1990-01-01" @default.
- W2080353507 modified "2023-10-17" @default.
- W2080353507 title "EMBRYOLOGICAL STUDY OF HERMINIUM MONORCHIS (ORCHIDACEAE) USING CONFOCAL SCANNING LASER MICROSCOPY" @default.
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- W2080353507 doi "https://doi.org/10.1002/j.1537-2197.1990.tb13535.x" @default.
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