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- W2080355320 abstract "Two alternatively spliced mRNAs (d- and l-MpLaeA) of a methyltransferase gene (MpLaeA) were identified from Monascus pilosus IFO4520 and its mutant MK-1. Alternative splicing of the MpLaeA pre-mRNA occurred in the 5′-untranslated region (5′-UTR). The alternative splicing patterns of MpLaeA were regulated by the fungal growth stage and the principal nutrients: that is, the short l-MpLaeA mRNA was a constitutive transcript at all growth stages and different carbon or nitrogen sources, but the glutamate and NaNO3 as main nitrogen source could up-regulate the long d-MpLaeA mRNA form. The long spliced 5′-UTR of d-MpLaeA blocked GFP expression in Escherichia coli, suggesting that d-MpLaeA mRNA was an ineffective spliced mRNA. Down-regulation of MpLaeA by transgenic antisense d-MpLaeA cDNA resulted in decreasing synthesis of monacolin K in M. pilosus. This suggested that the alternative splicing of MpLaeA mRNA might regulate the synthesis of monacolin K." @default.
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- W2080355320 date "2009-04-15" @default.
- W2080355320 modified "2023-09-23" @default.
- W2080355320 title "Development and Media Regulate Alternative Splicing of a Methyltransferase Pre-mRNA in Monascus pilosus" @default.
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- W2080355320 doi "https://doi.org/10.1021/jf9004109" @default.
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