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- W2080366279 abstract "Cholinergic interneurons are the only known source of acetylcholine in the rat nucleus accumbens (nAcb); yet there is little anatomical data about their mode of innervation and the origin of their excitatory drive. We characterized the cholinergic and thalamic innervations of nAcb with choline acetyltransferase (ChAT) immunocytochemistry and anterograde transport of Phaseolus vulgaris-leucoagglutinin (PHA-L) from the midline/intralaminar/paraventricular thalamic nuclei. The use of a monoclonal ChAT antiserum against whole rat ChAT protein allowed for an optimal visualization of the small dendritic branches and fine varicose axons of cholinergic interneurons. PHA-L-labeled thalamic afferents were heterogeneously distributed throughout the core and shell regions of nAcb, overlapping regionally with cholinergic somata and dendrites. At the ultrastructural level, several hundred single-section profiles of PHA-L and ChAT-labeled axon terminals were analyzed for morphology, synaptic frequency, and the nature of their synaptic targets. The cholinergic profiles were small and apposed to various neuronal elements, but rarely exhibited a synaptic membrane specialization (5% in single ultrathin sections). Stereological extrapolation indicated that less than 15% of these cholinergic varicosities were synaptic. The PHA-L-labeled profiles were comparatively large and often synaptic (37% in single ultrathin sections), making asymmetrical contacts primarily with dendritic spines (>90%). Stereological extrapolation indicated that all PHA-L-labeled terminals were synaptic. In double-labeled material, some PHA-L-labeled terminals were directly apposed to ChAT-labeled somata or dendrites, but synapses were never seen between the two types of elements. These observations demonstrate that the cholinergic innervation of rat nAcb is largely asynaptic. They confirm that the afferents from midline/intralaminar/paraventricular thalamic nuclei to rat nAcb synapse mostly on dendritic spines, presumably of medium spiny neurons, and suggest that the excitatory drive of nAcb cholinergic interneurons from thalamus is indirect, either via substance P release from recurrent collaterals of medium spiny neurons and/or by extrasynaptic diffusion of glutamate." @default.
- W2080366279 created "2016-06-24" @default.
- W2080366279 creator A5027306476 @default.
- W2080366279 creator A5040551439 @default.
- W2080366279 creator A5062535346 @default.
- W2080366279 date "2009-01-01" @default.
- W2080366279 modified "2023-10-16" @default.
- W2080366279 title "Cholinergic innervation and thalamic input in rat nucleus accumbens" @default.
- W2080366279 cites W1484597533 @default.
- W2080366279 cites W1543271211 @default.
- W2080366279 cites W1557320868 @default.
- W2080366279 cites W1595473599 @default.
- W2080366279 cites W1622774380 @default.
- W2080366279 cites W1776364276 @default.
- W2080366279 cites W1833549490 @default.
- W2080366279 cites W1878137681 @default.
- W2080366279 cites W1885050361 @default.
- W2080366279 cites W1910271824 @default.
- W2080366279 cites W1963674691 @default.
- W2080366279 cites W1964060360 @default.
- W2080366279 cites W1964104617 @default.
- W2080366279 cites W1964240411 @default.
- W2080366279 cites W1965123670 @default.
- W2080366279 cites W1966103661 @default.
- W2080366279 cites W1967875997 @default.
- W2080366279 cites W1969212890 @default.
- W2080366279 cites W1979032136 @default.
- W2080366279 cites W1981422377 @default.
- W2080366279 cites W1983348906 @default.
- W2080366279 cites W1985657042 @default.
- W2080366279 cites W1985938249 @default.
- W2080366279 cites W1986357584 @default.
- W2080366279 cites W1988829918 @default.
- W2080366279 cites W1993116569 @default.
- W2080366279 cites W1998195757 @default.
- W2080366279 cites W1998722012 @default.
- W2080366279 cites W2001181389 @default.
- W2080366279 cites W2001311556 @default.
- W2080366279 cites W2001754923 @default.
- W2080366279 cites W2003289804 @default.
- W2080366279 cites W2006457780 @default.
- W2080366279 cites W2007467910 @default.
- W2080366279 cites W2007480720 @default.
- W2080366279 cites W2009805692 @default.
- W2080366279 cites W2011964399 @default.
- W2080366279 cites W2012927321 @default.
- W2080366279 cites W2014320300 @default.
- W2080366279 cites W2014415095 @default.
- W2080366279 cites W2015161406 @default.
- W2080366279 cites W2017426413 @default.
- W2080366279 cites W2019645577 @default.
- W2080366279 cites W2020882643 @default.
- W2080366279 cites W2022254452 @default.
- W2080366279 cites W2024069005 @default.
- W2080366279 cites W2026633568 @default.
- W2080366279 cites W2028725530 @default.
- W2080366279 cites W2028980996 @default.
- W2080366279 cites W2029280276 @default.
- W2080366279 cites W2031219969 @default.
- W2080366279 cites W2032284808 @default.
- W2080366279 cites W2033298094 @default.
- W2080366279 cites W2033904402 @default.
- W2080366279 cites W2037607891 @default.
- W2080366279 cites W2039732315 @default.
- W2080366279 cites W2043313374 @default.
- W2080366279 cites W2043496808 @default.
- W2080366279 cites W2044533253 @default.
- W2080366279 cites W2045598952 @default.
- W2080366279 cites W2049547155 @default.
- W2080366279 cites W2049796382 @default.
- W2080366279 cites W2051961922 @default.
- W2080366279 cites W2053346549 @default.
- W2080366279 cites W2057135100 @default.
- W2080366279 cites W2057178098 @default.
- W2080366279 cites W2061583001 @default.
- W2080366279 cites W2063167199 @default.
- W2080366279 cites W2064856510 @default.
- W2080366279 cites W2065709888 @default.
- W2080366279 cites W2066099032 @default.
- W2080366279 cites W2066231624 @default.
- W2080366279 cites W2068480458 @default.
- W2080366279 cites W2071937933 @default.
- W2080366279 cites W2072300509 @default.
- W2080366279 cites W2072308972 @default.
- W2080366279 cites W2074887424 @default.
- W2080366279 cites W2077802026 @default.
- W2080366279 cites W2085230178 @default.
- W2080366279 cites W2085899483 @default.
- W2080366279 cites W2087593804 @default.
- W2080366279 cites W2089473311 @default.
- W2080366279 cites W2089765480 @default.
- W2080366279 cites W2092659452 @default.
- W2080366279 cites W2093983145 @default.
- W2080366279 cites W2094090855 @default.
- W2080366279 cites W2098607312 @default.
- W2080366279 cites W2100750550 @default.
- W2080366279 cites W2106001311 @default.
- W2080366279 cites W2108547360 @default.