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• W2080369903 abstract "A new food-grade expression/secretion vector for lactococci, pFMN30, was developed using an α-galactosidase gene (melA) of Lactobacillus plantarum as a selection marker. The 4.9-kb pFMN30 is a derivative of the lactococcal vector pMG36e containing a broad-host-range replicon of pWV01. In Lactococcus lactis, transformants carrying the vector were easily detectable by the appearance of a blue colony on a X-α-gal-containing medium and also by the growth on a medium containing melibiose as a sole carbon source. The expression/secretion vector was equipped with the controllable and strong nisA promoter. In addition, usp45 signal peptide was inserted for the efficient secretion of a foreign protein outside cells. The vector pFMN30 was used for the expression and secretion of α-amylase as a reporter gene, lacking a signal sequence derived from Bacillus licheniformis in L. lactis. These results show that the food-grade expression/secretion vector constructed in the present study could be used for the production of foreign proteins in L. lactis for the production food materials and also for the medicinal purposes." @default.
• W2080369903 created "2016-06-24" @default.
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• W2080369903 date "2006-08-01" @default.
• W2080369903 modified "2023-10-03" @default.
• W2080369903 title "A food-grade expression/secretion vector for Lactococcus lactis that uses an α-galactosidase gene as a selection marker" @default.
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• W2080369903 doi "https://doi.org/10.1016/j.fm.2005.06.003" @default.
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