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• W2080369970 abstract "Toxoplasma gondii can differentiate into tachyzoites or bradyzoites. To accelerate the investigation of bradyzoite differentiation mechanisms, we constructed a reporter parasite, PLK/DLUC_1C9, for a high-throughput assay. PLK/DLUC_1C9 expressed firefly luciferase under the bradyzoite-specific BAG1 promoter. Firefly luciferase activity was detected with a minimum of 102 parasites induced by pH 8.1. To normalize bradyzoite differentiation, PLK/DLUC_1C9 expressed Renilla luciferase under the parasite’s α-tubulin promoter. Renilla luciferase activity was detected with at least 102 parasites. By using PLK/DLUC_1C9 with this 96-well format screening system, we found that the protein kinase inhibitor analogs, bumped kinase inhibitors 1NM-PP1, 3MB-PP1, and 3BrB-PP1, had bradyzoite-inducing effects." @default.
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• W2080369970 date "2014-11-01" @default.
• W2080369970 modified "2023-10-17" @default.
• W2080369970 title "Microplate assay for screening Toxoplasma gondii bradyzoite differentiation with DUAL luciferase assay" @default.
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• W2080369970 doi "https://doi.org/10.1016/j.ab.2014.06.018" @default.
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