FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080370921> ?p ?o ?g. }

• W2080370921 endingPage "19" @default.
• W2080370921 startingPage "10" @default.
• W2080370921 abstract "Oleic (cis9-18:1), linoleic (cis9,cis12-18:2) and α-linolenic (cis9,cis12,cis15-18:3) acids are well described substrates of the Δ6-desaturase encoded by the mammalian fatty acid desaturase 2 (FADS2) gene. In addition, at least 9 other very structurally different fatty acids have been shown to be Δ6- or even Δ8-desaturated by the FADS2 protein. A better characterization of the substrate specificity of this enzyme is therefore needed. By using commercial cis9-18:1 and chemically synthesized cis12- and cis15-18:1 (sharing the n-6 double bond with 18:2 n-6 and the n-3 double bond with 18:3 n-3, respectively), we tried to decrypt the fatty acid structure driving the FADS2 substrate affinity. We first showed that both recombinant and native rat FADS2 were able to Δ6-desaturate not only the cis9- but also the cis12- and cis15-18:1 isomers. Next, the inhibitory effect of increasing concentrations of each 18:1 isomer was investigated in vitro on the Δ6-desaturation of α-linolenic acid. At equimolar inhibitor/substrate ratio (60 μM), the cis9-18:1 exhibited a significantly higher inhibition (25%) than the cis12- (8%) and cis15-18:1 (5%). This study shows that a single cis double bond in 12- or 15-position in 18:1 is enough to make them low Δ6-desaturable substrates. If a preexisting cis9-double bond is not absolutely required for the Δ6-desaturation of octadecenoic acids, its presence is however crucial to explain the higher enzyme affinity. Compared with oleic acid, the additional presence of a cis12-double bond in linoleic acid increased its inhibitory effect on the Δ6-desaturation of α-linolenic acid at low concentration (30 μM) but not at higher concentrations (60 and 120 μM). In this classification of the decreasing impact of the double bond when it comes closer to the methyl end of octadecenoic acids, the cis11-18:1 (cis-vaccenic acid) should be considered apart since it is itself not Δ6-desaturated but still a good competitive inhibitor of the α-linolenic acid Δ6-desaturation." @default.
• W2080370921 created "2016-06-24" @default.
• W2080370921 creator A5005733484 @default.
• W2080370921 creator A5007997759 @default.
• W2080370921 creator A5028675960 @default.
• W2080370921 creator A5035355633 @default.
• W2080370921 creator A5078512785 @default.
• W2080370921 creator A5089833359 @default.
• W2080370921 date "2015-04-01" @default.
• W2080370921 modified "2023-10-14" @default.
• W2080370921 title "Influence of the cis-9, cis-12 and cis-15 double bond position in octadecenoic acid (18:1) isomers on the rat FADS2-catalyzed Δ6-desaturation" @default.
• W2080370921 cites W1606867902 @default.
• W2080370921 cites W1772667072 @default.
• W2080370921 cites W1907473378 @default.
• W2080370921 cites W1966991669 @default.
• W2080370921 cites W1967390261 @default.
• W2080370921 cites W1968322809 @default.
• W2080370921 cites W1970862991 @default.
• W2080370921 cites W1972352783 @default.
• W2080370921 cites W1973895626 @default.
• W2080370921 cites W1974401020 @default.
• W2080370921 cites W1980420553 @default.
• W2080370921 cites W1984189444 @default.
• W2080370921 cites W1989133249 @default.
• W2080370921 cites W1996354611 @default.
• W2080370921 cites W2007749525 @default.
• W2080370921 cites W2021504392 @default.
• W2080370921 cites W2021754900 @default.
• W2080370921 cites W2032555155 @default.
• W2080370921 cites W2045716324 @default.
• W2080370921 cites W2046195246 @default.
• W2080370921 cites W2051745134 @default.
• W2080370921 cites W2059389236 @default.
• W2080370921 cites W2062551414 @default.
• W2080370921 cites W2069404154 @default.
• W2080370921 cites W2073338663 @default.
• W2080370921 cites W2077030170 @default.
• W2080370921 cites W2086823949 @default.
• W2080370921 cites W2089928956 @default.
• W2080370921 cites W2090063199 @default.
• W2080370921 cites W2090784961 @default.
• W2080370921 cites W2094103449 @default.
• W2080370921 cites W2095484074 @default.
• W2080370921 cites W2095536791 @default.
• W2080370921 cites W2105608546 @default.
• W2080370921 cites W2116130047 @default.
• W2080370921 cites W2130318680 @default.
• W2080370921 cites W2139560266 @default.
• W2080370921 cites W2147339290 @default.
• W2080370921 cites W2170539205 @default.
• W2080370921 cites W2182412427 @default.
• W2080370921 doi "https://doi.org/10.1016/j.chemphyslip.2015.02.001" @default.
• W2080370921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25701799" @default.
• W2080370921 hasPublicationYear "2015" @default.
• W2080370921 type Work @default.
• W2080370921 sameAs 2080370921 @default.
• W2080370921 citedByCount "8" @default.
• W2080370921 countsByYear W20803709212017 @default.
• W2080370921 countsByYear W20803709212018 @default.
• W2080370921 countsByYear W20803709212019 @default.
• W2080370921 countsByYear W20803709212020 @default.
• W2080370921 countsByYear W20803709212021 @default.
• W2080370921 crossrefType "journal-article" @default.
• W2080370921 hasAuthorship W2080370921A5005733484 @default.
• W2080370921 hasAuthorship W2080370921A5007997759 @default.
• W2080370921 hasAuthorship W2080370921A5028675960 @default.
• W2080370921 hasAuthorship W2080370921A5035355633 @default.
• W2080370921 hasAuthorship W2080370921A5078512785 @default.
• W2080370921 hasAuthorship W2080370921A5089833359 @default.
• W2080370921 hasConcept C111368507 @default.
• W2080370921 hasConcept C127313418 @default.
• W2080370921 hasConcept C178790620 @default.
• W2080370921 hasConcept C185592680 @default.
• W2080370921 hasConcept C19038510 @default.
• W2080370921 hasConcept C2776176469 @default.
• W2080370921 hasConcept C2777171753 @default.
• W2080370921 hasConcept C2777289219 @default.
• W2080370921 hasConcept C2777686832 @default.
• W2080370921 hasConcept C2778915593 @default.
• W2080370921 hasConcept C2780809830 @default.
• W2080370921 hasConcept C2781403372 @default.
• W2080370921 hasConcept C543025807 @default.
• W2080370921 hasConcept C55493867 @default.
• W2080370921 hasConcept C68381374 @default.
• W2080370921 hasConcept C71240020 @default.
• W2080370921 hasConceptScore W2080370921C111368507 @default.
• W2080370921 hasConceptScore W2080370921C127313418 @default.
• W2080370921 hasConceptScore W2080370921C178790620 @default.
• W2080370921 hasConceptScore W2080370921C185592680 @default.
• W2080370921 hasConceptScore W2080370921C19038510 @default.
• W2080370921 hasConceptScore W2080370921C2776176469 @default.
• W2080370921 hasConceptScore W2080370921C2777171753 @default.
• W2080370921 hasConceptScore W2080370921C2777289219 @default.
• W2080370921 hasConceptScore W2080370921C2777686832 @default.
• W2080370921 hasConceptScore W2080370921C2778915593 @default.
• W2080370921 hasConceptScore W2080370921C2780809830 @default.
• W2080370921 hasConceptScore W2080370921C2781403372 @default.
• W2080370921 hasConceptScore W2080370921C543025807 @default.

• next