FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080378360> ?p ?o ?g. }

• W2080378360 endingPage "199" @default.
• W2080378360 startingPage "179" @default.
• W2080378360 abstract "1. Voltage-activated currents have been recorded from cerebellar granule neurones in explant cultures from young rats (1-9 days old). Cells were examined with whole-cell patch-clamp methods. Depolarizing pulses from a pre-pulse potential of -100 mV evoked a rapidly activated transient inward current, and an outward current which decayed in two phases. The ionic dependence, kinetics and pharmacological properties of these currents have been studied. 2. Peak inward Na+ currents in cells from 7-day-old rats were in the range 350-450 pA. No evidence was found for the presence of calcium currents. Thus, inward current was unchanged in zero Ca2+, 1 mM-EGTA solution. No inward current was obtained in medium containing 10 mM-Ba2+ and tetrodotoxin (TTX). Supplementing the pipette (i.e. intracellular) solution with Mg-ATP did not reveal any Ca2+ current. 3. Depolarizing steps (from -100 mV) in TTX-containing solution gave an early transient outward current and a late outward current. The transient current resembled IA described in other cells, and reversed close to EK in both normal and elevated potassium concentrations, indicating that K+ is the predominant charge carrier. Depolarizing steps from -50 mV failed to give a transient outward current, and gave only a slowly rising current which resembled the late potassium current, IK. 4. Inactivation of the transient current was examined by applying test depolarizations from increasingly negative pre-pulse potentials (-50 to -120 mV): half-inactivation occurred at -72 mV. Transient outward currents decayed exponentially with time constants, tau, of 7.3-25.3 ms at 0 mV. The time course of removal of inactivation in cells held at -50 mV, and given increasingly long pre-pulses to -100 mV, was exponential with tau = 35 ms. 5. Both transient and late outward currents were reversibly abolished by addition to the bathing medium of 10 mM-Ba2+ or 1 mM-quinine. Outward K+ current was not dependent on external calcium. Tetraethylammonium (20 mM) selectively reduced the late outward current; the peak transient current was reduced by less than 20%. 4-Aminopyridine (2 mM) showed little selectivity between transient and late outward currents. 6. It is concluded that cerebellar granule cells from young rats possess voltage-activated inward Na+ current as well as two types of K+ current, IA and IK. In terms of neuronal functioning, the properties of the transient outward current may confer a role in regulating excitability and in repolarization, but a definitive statement will require knowledge of the cellular location and relative densities of channels in granule cells in vivo." @default.
• W2080378360 created "2016-06-24" @default.
• W2080378360 creator A5060588445 @default.
• W2080378360 creator A5069305015 @default.
• W2080378360 creator A5087490351 @default.
• W2080378360 date "1989-07-01" @default.
• W2080378360 modified "2023-09-26" @default.
• W2080378360 title "Voltage-activated membrane currents in rat cerebellar granule neurones." @default.
• W2080378360 cites W1487165586 @default.
• W2080378360 cites W1487954084 @default.
• W2080378360 cites W1520942021 @default.
• W2080378360 cites W1540820991 @default.
• W2080378360 cites W1636078186 @default.
• W2080378360 cites W1968222822 @default.
• W2080378360 cites W1970069829 @default.
• W2080378360 cites W1970398364 @default.
• W2080378360 cites W1972046379 @default.
• W2080378360 cites W1972371527 @default.
• W2080378360 cites W1974846950 @default.
• W2080378360 cites W1978317061 @default.
• W2080378360 cites W1993043025 @default.
• W2080378360 cites W1993217378 @default.
• W2080378360 cites W1993909750 @default.
• W2080378360 cites W1997511216 @default.
• W2080378360 cites W1999144393 @default.
• W2080378360 cites W2009667219 @default.
• W2080378360 cites W2011921542 @default.
• W2080378360 cites W2015426350 @default.
• W2080378360 cites W2020023795 @default.
• W2080378360 cites W2028547793 @default.
• W2080378360 cites W2031920967 @default.
• W2080378360 cites W2031998157 @default.
• W2080378360 cites W2043338542 @default.
• W2080378360 cites W2053637259 @default.
• W2080378360 cites W2059612988 @default.
• W2080378360 cites W2081785456 @default.
• W2080378360 cites W2083602054 @default.
• W2080378360 cites W2084679742 @default.
• W2080378360 cites W2093724234 @default.
• W2080378360 cites W2100548210 @default.
• W2080378360 cites W2124300639 @default.
• W2080378360 cites W2141056714 @default.
• W2080378360 cites W2288331821 @default.
• W2080378360 cites W2294112213 @default.
• W2080378360 doi "https://doi.org/10.1113/jphysiol.1989.sp017683" @default.
• W2080378360 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1189137" @default.
• W2080378360 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2558168" @default.
• W2080378360 hasPublicationYear "1989" @default.
• W2080378360 type Work @default.
• W2080378360 sameAs 2080378360 @default.
• W2080378360 citedByCount "106" @default.
• W2080378360 countsByYear W20803783602012 @default.
• W2080378360 countsByYear W20803783602013 @default.
• W2080378360 countsByYear W20803783602014 @default.
• W2080378360 countsByYear W20803783602016 @default.
• W2080378360 countsByYear W20803783602020 @default.
• W2080378360 countsByYear W20803783602021 @default.
• W2080378360 crossrefType "journal-article" @default.
• W2080378360 hasAuthorship W2080378360A5060588445 @default.
• W2080378360 hasAuthorship W2080378360A5069305015 @default.
• W2080378360 hasAuthorship W2080378360A5087490351 @default.
• W2080378360 hasBestOaLocation W20803783601 @default.
• W2080378360 hasConcept C100837961 @default.
• W2080378360 hasConcept C101404756 @default.
• W2080378360 hasConcept C12554922 @default.
• W2080378360 hasConcept C147944092 @default.
• W2080378360 hasConcept C169760540 @default.
• W2080378360 hasConcept C170493617 @default.
• W2080378360 hasConcept C178790620 @default.
• W2080378360 hasConcept C181911157 @default.
• W2080378360 hasConcept C185263204 @default.
• W2080378360 hasConcept C185592680 @default.
• W2080378360 hasConcept C2776741303 @default.
• W2080378360 hasConcept C2778071365 @default.
• W2080378360 hasConcept C4141045 @default.
• W2080378360 hasConcept C517785266 @default.
• W2080378360 hasConcept C55493867 @default.
• W2080378360 hasConcept C86803240 @default.
• W2080378360 hasConceptScore W2080378360C100837961 @default.
• W2080378360 hasConceptScore W2080378360C101404756 @default.
• W2080378360 hasConceptScore W2080378360C12554922 @default.
• W2080378360 hasConceptScore W2080378360C147944092 @default.
• W2080378360 hasConceptScore W2080378360C169760540 @default.
• W2080378360 hasConceptScore W2080378360C170493617 @default.
• W2080378360 hasConceptScore W2080378360C178790620 @default.
• W2080378360 hasConceptScore W2080378360C181911157 @default.
• W2080378360 hasConceptScore W2080378360C185263204 @default.
• W2080378360 hasConceptScore W2080378360C185592680 @default.
• W2080378360 hasConceptScore W2080378360C2776741303 @default.
• W2080378360 hasConceptScore W2080378360C2778071365 @default.
• W2080378360 hasConceptScore W2080378360C4141045 @default.
• W2080378360 hasConceptScore W2080378360C517785266 @default.
• W2080378360 hasConceptScore W2080378360C55493867 @default.
• W2080378360 hasConceptScore W2080378360C86803240 @default.
• W2080378360 hasIssue "1" @default.
• W2080378360 hasLocation W20803783601 @default.
• W2080378360 hasLocation W20803783602 @default.
• W2080378360 hasLocation W20803783603 @default.

• next