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• W2080392883 abstract "Electron probe X-ray microanalysis of fracture faces of quench frozen hydrated bulk samples of Lemna minor fronds exposed to high levels of Zn (300 µM) has shown the presence of globular deposits up to 1 µm in diameter containing Zn, K and P in parenchyma cells of mature fronds, and Zn, K, Mg and P in immature parenchyma cells of daughter fronds (or turions). Although this method of direct analysis of bulk samples does not lend itself to a quantitative comparison of number and composition of globules in Zn-tolerant and Zn-sensitive clones of Lemna minor, it has the advantages of simplicity of specimen preparation, rapid detection of Zn-P-containing globules, and avoidance of diffusional artifacts. Comparisons with prepared K Mg phytate and Zn standards, and additional data obtained by X-ray microanalysis of freeze-substituted thin sections, established the elemental proportions of Zn, Mg, K and Ca relative to P, which indicate that generally up to 8 (molar ratio of Zn to phytic acid = 4) and sometimes up to 12 (molar ratio of Zn to phytic acid = 6) valencies can be occupied by Zn and up to 4 by K and/or Mg. Some of the Ca that appears to be associated with globules may result from elemental redistribution during the process of freeze-substitution especially when Ca oxalate crystals are present in neighbouring raphide cells. X-ray microanalysis provided evidence for the absence of Zn from these raphides." @default.
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• W2080392883 date "1990-12-01" @default.
• W2080392883 modified "2023-10-16" @default.
• W2080392883 title "Zinc tolerance and the binding of zinc as zinc phytate in Lemna minor. X-ray microanalytical evidence" @default.
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• W2080392883 doi "https://doi.org/10.1016/s0176-1617(11)80070-2" @default.
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