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• W2080402521 endingPage "5497" @default.
• W2080402521 startingPage "5493" @default.
• W2080402521 abstract "A new expression plasmid containing the fla operon promoter and a staphylococcal chloramphenicol resistance gene, was constructed to help assess the role of fliG in Treponema denticola motility. Deletion of fliG resulted in a nonmotile mutant with a markedly decreased number of flagellar filaments. Wild-type fliG genes from T. denticola and from Treponema pallidum were cloned into this expression plasmid. In both cases, the gene restored the ability of the mutant to gyrate its cell ends and enabled colony spreading in agarose. This shuttle plasmid enables high-level expression of genes in T. denticola and possesses an efficient selectable marker that provides a new tool for treponemal genetics." @default.
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• W2080402521 date "2004-09-01" @default.
• W2080402521 modified "2023-10-10" @default.
• W2080402521 title "Development of a Novel Chloramphenicol Resistance Expression Plasmid Used for Genetic Complementation of a <i>fliG</i> Deletion Mutant in <i>Treponema denticola</i>" @default.
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• W2080402521 doi "https://doi.org/10.1128/iai.72.9.5493-5497.2004" @default.
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