FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080407409> ?p ?o ?g. }

• W2080407409 endingPage "33" @default.
• W2080407409 startingPage "25" @default.
• W2080407409 abstract "Objective Pronucleating nonmucin glycoproteins in human cholesterol and black gallstones were isolated and identified. Summary Background Data Gallbladder bile contains nonmucin glycoproteins that are pronucteating of cholesterol monohydrate crystals. Little is known about the presence or activity of these proteins within gallstones. Methods Nonmucin glycoproteins were isolated from single cholesterol (n = 8), multiple cholesterol (n = 8), and black pigment (n = 8) gallstones by concanavalin A lectin-affinity chromatography. The proteins were separated by sodium dodecyl sulfate gradient electrophoresis. Western blot analysis was performed for Fab immunoglobulin fragments, and heavy chains from the immunoglobulin G, A, E, and M subclasses. A crystal observation time assay was performed on the combination of isolated nonmucin glycoproteins from gallstones and isolated Fab fragments. Results Nonmucin glycoproteins of molecular weights 10, 15, 17, 22, 28, and 208 kD were identified in gallstones. These six nonmucin glycoproteins shortened the crystal observation time by more than 50% (p < 0.01) compared with model bile. Western blot analysis confirmed the identity of the 22-and 28-kD proteins as immunoglobulin Fab fragments. These were seen in all gallstones, irrespective of the gallstone type. The isolated Fab 28-kD fragment from the gallstones of 23 patients shortened the extrapolated crystal observation time by 78% (p < 0.01). However, commercially available Fab fragments had no effect on either cholesterol crystal appearance or growth. Conclusions Nonmucin glycoproteins that are pronucleating for cholesterol monohydrate crystals are also found in human cholesterol and black pigment gallstones. Fab immunoglobulin fragments were found in all gallstones irrespective of the gallstone type. Fab immunoglobulin fragments from gallstones shortened the crystal observation time but not crystal growth or total crystal content compared with model bile or commercially available Fab Fragments. These data suggest that an antigen-immune (Fab) complex may contribute to cholesterol crystal function." @default.
• W2080407409 created "2016-06-24" @default.
• W2080407409 creator A5011555598 @default.
• W2080407409 creator A5012771182 @default.
• W2080407409 creator A5051782291 @default.
• W2080407409 creator A5051851456 @default.
• W2080407409 creator A5082370453 @default.
• W2080407409 date "1994-01-01" @default.
• W2080407409 modified "2023-09-25" @default.
• W2080407409 title "Human Gallstones Contain Pronucleating Nonmucin Glycoproteins That are Immunoglobulins" @default.
• W2080407409 cites W1562555406 @default.
• W2080407409 cites W1726603647 @default.
• W2080407409 cites W1769938989 @default.
• W2080407409 cites W1798682103 @default.
• W2080407409 cites W1895325632 @default.
• W2080407409 cites W1969242642 @default.
• W2080407409 cites W1976518863 @default.
• W2080407409 cites W1983297500 @default.
• W2080407409 cites W2010497181 @default.
• W2080407409 cites W2018043410 @default.
• W2080407409 cites W2018289835 @default.
• W2080407409 cites W2019968787 @default.
• W2080407409 cites W2024753390 @default.
• W2080407409 cites W2033756350 @default.
• W2080407409 cites W2034938568 @default.
• W2080407409 cites W2056474881 @default.
• W2080407409 cites W2066787005 @default.
• W2080407409 cites W2068689577 @default.
• W2080407409 cites W2084472201 @default.
• W2080407409 cites W2100837269 @default.
• W2080407409 cites W2158190765 @default.
• W2080407409 cites W2183433436 @default.
• W2080407409 cites W2326184548 @default.
• W2080407409 cites W75760083 @default.
• W2080407409 doi "https://doi.org/10.1097/00000658-199401000-00005" @default.
• W2080407409 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1243086" @default.
• W2080407409 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8297172" @default.
• W2080407409 hasPublicationYear "1994" @default.
• W2080407409 type Work @default.
• W2080407409 sameAs 2080407409 @default.
• W2080407409 citedByCount "17" @default.
• W2080407409 countsByYear W20804074092014 @default.
• W2080407409 crossrefType "journal-article" @default.
• W2080407409 hasAuthorship W2080407409A5011555598 @default.
• W2080407409 hasAuthorship W2080407409A5012771182 @default.
• W2080407409 hasAuthorship W2080407409A5051782291 @default.
• W2080407409 hasAuthorship W2080407409A5051851456 @default.
• W2080407409 hasAuthorship W2080407409A5082370453 @default.
• W2080407409 hasBestOaLocation W20804074092 @default.
• W2080407409 hasConcept C104317684 @default.
• W2080407409 hasConcept C108625454 @default.
• W2080407409 hasConcept C126322002 @default.
• W2080407409 hasConcept C153911025 @default.
• W2080407409 hasConcept C159654299 @default.
• W2080407409 hasConcept C19869320 @default.
• W2080407409 hasConcept C202751555 @default.
• W2080407409 hasConcept C203014093 @default.
• W2080407409 hasConcept C2776415932 @default.
• W2080407409 hasConcept C2777148285 @default.
• W2080407409 hasConcept C2778163477 @default.
• W2080407409 hasConcept C2779388726 @default.
• W2080407409 hasConcept C55493867 @default.
• W2080407409 hasConcept C71924100 @default.
• W2080407409 hasConcept C86803240 @default.
• W2080407409 hasConceptScore W2080407409C104317684 @default.
• W2080407409 hasConceptScore W2080407409C108625454 @default.
• W2080407409 hasConceptScore W2080407409C126322002 @default.
• W2080407409 hasConceptScore W2080407409C153911025 @default.
• W2080407409 hasConceptScore W2080407409C159654299 @default.
• W2080407409 hasConceptScore W2080407409C19869320 @default.
• W2080407409 hasConceptScore W2080407409C202751555 @default.
• W2080407409 hasConceptScore W2080407409C203014093 @default.
• W2080407409 hasConceptScore W2080407409C2776415932 @default.
• W2080407409 hasConceptScore W2080407409C2777148285 @default.
• W2080407409 hasConceptScore W2080407409C2778163477 @default.
• W2080407409 hasConceptScore W2080407409C2779388726 @default.
• W2080407409 hasConceptScore W2080407409C55493867 @default.
• W2080407409 hasConceptScore W2080407409C71924100 @default.
• W2080407409 hasConceptScore W2080407409C86803240 @default.
• W2080407409 hasIssue "1" @default.
• W2080407409 hasLocation W20804074091 @default.
• W2080407409 hasLocation W20804074092 @default.
• W2080407409 hasLocation W20804074093 @default.
• W2080407409 hasOpenAccess W2080407409 @default.
• W2080407409 hasPrimaryLocation W20804074091 @default.
• W2080407409 hasRelatedWork W1906133223 @default.
• W2080407409 hasRelatedWork W1994250600 @default.
• W2080407409 hasRelatedWork W2011981865 @default.
• W2080407409 hasRelatedWork W2042170604 @default.
• W2080407409 hasRelatedWork W2185831673 @default.
• W2080407409 hasRelatedWork W2315045608 @default.
• W2080407409 hasRelatedWork W2327967824 @default.
• W2080407409 hasRelatedWork W2358275342 @default.
• W2080407409 hasRelatedWork W2413441354 @default.
• W2080407409 hasRelatedWork W2419143163 @default.
• W2080407409 hasVolume "219" @default.
• W2080407409 isParatext "false" @default.
• W2080407409 isRetracted "false" @default.

• next