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• W2080453586 abstract "The aminoglycoside kasugamycin, which has previously been shown to inhibit initiation of protein biosynthesis in vitro, also affects translational accuracy in vitro. This is deduced from the observation that the drug decreases the incorporation of histidine relative to alanine into the coat protein of phage MS2, the gene of which is devoid of histidine codons. The read‐through of the MS2 coat cistron, due to frameshifts in vitro, is also suppressed by the antibiotic. In contrast, streptomycin enhances histidine incorporation and read‐through in this system. The effects of kasugamycin take place at concentrations that do not inhibit coat protein biosynthesis. Kasugamycin‐resistant mutants ( ksgA ) lacking dimethylation of two adjacent adenosines in 16 S ribosomal RNA, show an increased leakiness of nonsense and frameshift mutants (in the absence of antibiotic). They are therefore phenotypically similar to previously described ribosomal ambiguity mutants ( ram )." @default.
• W2080453586 created "2016-06-24" @default.
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• W2080453586 date "1984-11-05" @default.
• W2080453586 modified "2023-10-15" @default.
• W2080453586 title "Increased translational fidelity caused by the antibiotic kasugamycin and ribosomal ambiguity in mutants harbouring the <i>ksgA</i> gene" @default.
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• W2080453586 doi "https://doi.org/10.1016/0014-5793(84)80994-1" @default.
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