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• W2080455319 endingPage "455" @default.
• W2080455319 startingPage "445" @default.
• W2080455319 abstract "The picosecond absorption kinetics of light adapted bacteriorhodops in (BR) are reported for water and glycerol-water suspensions of purple membrane from Halobacieriwn hulobium at different temperatures (77-300 K) and pH (5-10). It is shown that a photoproduct of BR, called P-BR (pseudo-bacteriorhodopsin). is responsible for the 16 ± 2 ps relaxation lifetime observed under steady state irradiation (i.e. with a train of ps pulses) at room temperature. At 77 K the absorption recovery lifetime is 62 ± 5 ps in good agreement with previous fluorescence lifetime studies. The observed signal is very sensitive to the sample flow rate and at the highest flow rates a fast absorbance increase (≤ 2 ps) is observed at λ > 620 nm. while at 576 nm a similarly fast absorption recovery alter bleaching is observed. These results imply that the reaction BR → K-BR occurs within 2 ps. In order to explain the simultaneous formation of P-BR and K-BR at 77 K. a model for the primary events including a traus-cis isomerization and a protein relaxation about the chromophore is suggested. The same model can also be used in explaining the simultaneous formation of batho- and hypsorhodopsin in some rhodopsins. Finally, a scheme for the last steps in the photocycle of bacteriorhodopsin including protonation, protein relaxation and cis-trans isomerization is proposed" @default.
• W2080455319 created "2016-06-24" @default.
• W2080455319 creator A5004159977 @default.
• W2080455319 creator A5006671446 @default.
• W2080455319 date "1983-04-01" @default.
• W2080455319 modified "2023-09-26" @default.
• W2080455319 title "PICOSECOND KINETICS and A MODEL FOR THE PRIMARY EVENTS OF BACTERIORHODOPSIN" @default.
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• W2080455319 doi "https://doi.org/10.1111/j.1751-1097.1983.tb04498.x" @default.
• W2080455319 hasPublicationYear "1983" @default.
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