FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080455900> ?p ?o ?g. }

• W2080455900 endingPage "309" @default.
• W2080455900 startingPage "303" @default.
• W2080455900 abstract "This study examines the effects of adrenergic ligands, cholera toxin, forskolin, and varying levels of beta(2) adrenergic receptors (beta(2)AR) on the cellular distribution of Gs(alpha) subunits in CHO cells. Localization of Gs(alpha) was evaluated by confocal microscopy and beta(2)AR-mediated signalling was assessed by adenylyl cyclase (AC) activity. In cells expressing 0.2 pmol/mg protein beta(2)ARs (WT18), the localization of Gs(alpha) subunit was restricted to the plasma membrane region. Isoproterenol (ISO), cholera toxin or forskolin elicited redistribution of cellular Gs(alpha) so that Gs(alpha) appeared as intense spots throughout the plasma membrane as well as the cytoplasm. Exposure to a neutral beta(2)AR antagonist, alprenolol, prevented the ISO-stimulated Gs(alpha) translocation from peripheral to inner cytoplasm. In cells expressing high level of beta(2)ARs (8.2 pmol/mg) (WT4), basal and ISO-stimulated AC activities were significantly elevated when compared to the values detected in WT18 clone, suggesting a positive correlation between receptor expression and receptor-mediated signalling. Basal Gs(alpha) distribution in this group was similar to that observed in ISO-, cholera toxin-, or forskolin-stimulated WT18 clone. ISO, cholera toxin, or forskolin did not change the distribution of Gs(alpha) significantly when tested in WT4 clone. No difference in the cellular level of Gs(alpha) protein between WT18 and WT4 clones was detected. Alprenolol did not affect the distribution of Gs(alpha) in WT4 clone. ICI 118,551, a negative beta(2)AR antagonist, altered Gs(alpha) distribution from a dispersed basal pattern to a membrane-confined pattern. The latter appearance was similar to that observed in unstimulated WT18 clone. Taken together, these data suggest that: (1) enhanced beta(2)AR-Gs(alpha) coupling induced by agonist stimulation or by increased expression of beta(2)ARs remodel the cellular distribution of Gs(alpha); (2) the alteration in Gs(alpha) distribution induced by beta(2)AR overexpression provides evidence for agonist-independent interaction of beta(2)AR and Gs(alpha), that can be inhibited by a negative antagonist but not by a neutral antagonist; and (3) forskolin influences the activity state of Gs(alpha) that displays a Gs(alpha) distribution pattern comparable to that observed when Gs(alpha) is activated via beta(2)AR stimulation or directly by cholera toxin." @default.
• W2080455900 created "2016-06-24" @default.
• W2080455900 creator A5031247002 @default.
• W2080455900 creator A5038225745 @default.
• W2080455900 creator A5050127758 @default.
• W2080455900 creator A5060879958 @default.
• W2080455900 creator A5076737326 @default.
• W2080455900 creator A5079111087 @default.
• W2080455900 date "2000-05-01" @default.
• W2080455900 modified "2023-09-25" @default.
• W2080455900 title "The effects of agonist stimulation and β2-adrenergic receptor level on cellular distribution of Gsα protein" @default.
• W2080455900 cites W1489775551 @default.
• W2080455900 cites W1499138436 @default.
• W2080455900 cites W1517947547 @default.
• W2080455900 cites W1530944849 @default.
• W2080455900 cites W1571519589 @default.
• W2080455900 cites W1889813890 @default.
• W2080455900 cites W1934687632 @default.
• W2080455900 cites W1981047025 @default.
• W2080455900 cites W1999279782 @default.
• W2080455900 cites W2017451004 @default.
• W2080455900 cites W2020592894 @default.
• W2080455900 cites W2037300971 @default.
• W2080455900 cites W2051034190 @default.
• W2080455900 cites W2055185804 @default.
• W2080455900 cites W2073040909 @default.
• W2080455900 cites W2080875376 @default.
• W2080455900 cites W2100837269 @default.
• W2080455900 cites W2165603941 @default.
• W2080455900 cites W2338968541 @default.
• W2080455900 cites W4293247451 @default.
• W2080455900 cites W1788108748 @default.
• W2080455900 doi "https://doi.org/10.1016/s0898-6568(00)00071-1" @default.
• W2080455900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10822171" @default.
• W2080455900 hasPublicationYear "2000" @default.
• W2080455900 type Work @default.
• W2080455900 sameAs 2080455900 @default.
• W2080455900 citedByCount "4" @default.
• W2080455900 crossrefType "journal-article" @default.
• W2080455900 hasAuthorship W2080455900A5031247002 @default.
• W2080455900 hasAuthorship W2080455900A5038225745 @default.
• W2080455900 hasAuthorship W2080455900A5050127758 @default.
• W2080455900 hasAuthorship W2080455900A5060879958 @default.
• W2080455900 hasAuthorship W2080455900A5076737326 @default.
• W2080455900 hasAuthorship W2080455900A5079111087 @default.
• W2080455900 hasConcept C126322002 @default.
• W2080455900 hasConcept C134018914 @default.
• W2080455900 hasConcept C153911025 @default.
• W2080455900 hasConcept C170493617 @default.
• W2080455900 hasConcept C176303035 @default.
• W2080455900 hasConcept C24998067 @default.
• W2080455900 hasConcept C2777006276 @default.
• W2080455900 hasConcept C2778938600 @default.
• W2080455900 hasConcept C2779178603 @default.
• W2080455900 hasConcept C2779276759 @default.
• W2080455900 hasConcept C2780275434 @default.
• W2080455900 hasConcept C55493867 @default.
• W2080455900 hasConcept C71924100 @default.
• W2080455900 hasConcept C80631254 @default.
• W2080455900 hasConcept C86803240 @default.
• W2080455900 hasConceptScore W2080455900C126322002 @default.
• W2080455900 hasConceptScore W2080455900C134018914 @default.
• W2080455900 hasConceptScore W2080455900C153911025 @default.
• W2080455900 hasConceptScore W2080455900C170493617 @default.
• W2080455900 hasConceptScore W2080455900C176303035 @default.
• W2080455900 hasConceptScore W2080455900C24998067 @default.
• W2080455900 hasConceptScore W2080455900C2777006276 @default.
• W2080455900 hasConceptScore W2080455900C2778938600 @default.
• W2080455900 hasConceptScore W2080455900C2779178603 @default.
• W2080455900 hasConceptScore W2080455900C2779276759 @default.
• W2080455900 hasConceptScore W2080455900C2780275434 @default.
• W2080455900 hasConceptScore W2080455900C55493867 @default.
• W2080455900 hasConceptScore W2080455900C71924100 @default.
• W2080455900 hasConceptScore W2080455900C80631254 @default.
• W2080455900 hasConceptScore W2080455900C86803240 @default.
• W2080455900 hasIssue "5" @default.
• W2080455900 hasLocation W20804559001 @default.
• W2080455900 hasLocation W20804559002 @default.
• W2080455900 hasOpenAccess W2080455900 @default.
• W2080455900 hasPrimaryLocation W20804559001 @default.
• W2080455900 hasRelatedWork W1977982331 @default.
• W2080455900 hasRelatedWork W2015286298 @default.
• W2080455900 hasRelatedWork W2017506957 @default.
• W2080455900 hasRelatedWork W2040451697 @default.
• W2080455900 hasRelatedWork W2075215458 @default.
• W2080455900 hasRelatedWork W2080455900 @default.
• W2080455900 hasRelatedWork W2104880421 @default.
• W2080455900 hasRelatedWork W2135734557 @default.
• W2080455900 hasRelatedWork W2194478523 @default.
• W2080455900 hasRelatedWork W2626456589 @default.
• W2080455900 hasVolume "12" @default.
• W2080455900 isParatext "false" @default.
• W2080455900 isRetracted "false" @default.
• W2080455900 magId "2080455900" @default.
• W2080455900 workType "article" @default.