FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080469723> ?p ?o ?g. }

• W2080469723 endingPage "e0122954" @default.
• W2080469723 startingPage "e0122954" @default.
• W2080469723 abstract "Multipotent mesenchymal stromal cells (MSC) have attracted interest for their cytotherapeutic potential, partly due to their immunomodulatory abilities. The aim of this study was to test the robustness of our equine cord blood (CB) MSC isolation protocol, to characterize the CB-MSC before and after cryopreservation, and to evaluate their immunosuppressive phenotype. We hypothesized that MSC can be consistently isolated from equine CB, have unique and reproducible marker expression and in vitro suppress lymphoproliferation. Preliminary investigation of constitutive cytoplasmic Toll-like receptor (TLR) 3 and 4 expression was also preformed due to their possible association with anti- or pro-inflammatory MSC phenotypes, respectively. Surface markers were assessed for antigen and mRNA expression by flow cytometry and quantitative polymerase chain reaction (qPCR). Immunomodulatory properties were evaluated in mixed lymphocyte reaction assays, and TLR3 and TLR4 expression were measured by qPCR and immunocytochemistry (ICC). CB-MSC were isolated from each off nine cord blood samples. CB-MSC highly expressed CD29, CD44, CD90, and lacked or had low expression of major histocompatibility complex (MHC) class I, MHC-II, CD4, CD8, CD11a/18 and CD73 before and after cryopreservation. CB-MSC suppressed in vitro lymphoproliferation and constitutively expressed TLR4. Our findings confirmed CB as a reliable MSC source, provides an association of surface marker phenotype and mRNA expression and suggest anti-inflammatory properties of CB-MSC. The relationship between TLRs and lymphocyte function warrants further investigation." @default.
• W2080469723 created "2016-06-24" @default.
• W2080469723 creator A5027995244 @default.
• W2080469723 creator A5036009102 @default.
• W2080469723 creator A5071612808 @default.
• W2080469723 creator A5074001179 @default.
• W2080469723 date "2015-04-22" @default.
• W2080469723 modified "2023-10-18" @default.
• W2080469723 title "Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells" @default.
• W2080469723 cites W1537881171 @default.
• W2080469723 cites W1577577364 @default.
• W2080469723 cites W1748891225 @default.
• W2080469723 cites W1783122597 @default.
• W2080469723 cites W1878620955 @default.
• W2080469723 cites W1898199407 @default.
• W2080469723 cites W1902093698 @default.
• W2080469723 cites W1964477864 @default.
• W2080469723 cites W1966659632 @default.
• W2080469723 cites W1969281312 @default.
• W2080469723 cites W1970632857 @default.
• W2080469723 cites W1982334709 @default.
• W2080469723 cites W1982408656 @default.
• W2080469723 cites W1985061684 @default.
• W2080469723 cites W1993314665 @default.
• W2080469723 cites W1994284905 @default.
• W2080469723 cites W2001472131 @default.
• W2080469723 cites W2009626854 @default.
• W2080469723 cites W2013918759 @default.
• W2080469723 cites W2014988196 @default.
• W2080469723 cites W2015162024 @default.
• W2080469723 cites W2025371271 @default.
• W2080469723 cites W2027375740 @default.
• W2080469723 cites W2036348189 @default.
• W2080469723 cites W2039092408 @default.
• W2080469723 cites W2041923443 @default.
• W2080469723 cites W2043536914 @default.
• W2080469723 cites W2047003816 @default.
• W2080469723 cites W2055358063 @default.
• W2080469723 cites W2058473775 @default.
• W2080469723 cites W2060676927 @default.
• W2080469723 cites W2064927636 @default.
• W2080469723 cites W2071080830 @default.
• W2080469723 cites W2073024911 @default.
• W2080469723 cites W2076326164 @default.
• W2080469723 cites W2078457908 @default.
• W2080469723 cites W2090273849 @default.
• W2080469723 cites W2090886213 @default.
• W2080469723 cites W2092779633 @default.
• W2080469723 cites W2102051218 @default.
• W2080469723 cites W2107277218 @default.
• W2080469723 cites W2108440267 @default.
• W2080469723 cites W2109670212 @default.
• W2080469723 cites W2116500923 @default.
• W2080469723 cites W2119194984 @default.
• W2080469723 cites W2124176539 @default.
• W2080469723 cites W2124932841 @default.
• W2080469723 cites W2127380828 @default.
• W2080469723 cites W2127480485 @default.
• W2080469723 cites W2137797941 @default.
• W2080469723 cites W2138374638 @default.
• W2080469723 cites W2140413558 @default.
• W2080469723 cites W2144046875 @default.
• W2080469723 cites W2145769955 @default.
• W2080469723 cites W2150753734 @default.
• W2080469723 cites W2153949260 @default.
• W2080469723 cites W2155881004 @default.
• W2080469723 cites W2160978572 @default.
• W2080469723 cites W2164841992 @default.
• W2080469723 cites W2168420558 @default.
• W2080469723 cites W2172234231 @default.
• W2080469723 cites W2192080449 @default.
• W2080469723 cites W2269778809 @default.
• W2080469723 cites W2312512876 @default.
• W2080469723 cites W3145620386 @default.
• W2080469723 doi "https://doi.org/10.1371/journal.pone.0122954" @default.
• W2080469723 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4406608" @default.
• W2080469723 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25902064" @default.
• W2080469723 hasPublicationYear "2015" @default.
• W2080469723 type Work @default.
• W2080469723 sameAs 2080469723 @default.
• W2080469723 citedByCount "38" @default.
• W2080469723 countsByYear W20804697232016 @default.
• W2080469723 countsByYear W20804697232017 @default.
• W2080469723 countsByYear W20804697232018 @default.
• W2080469723 countsByYear W20804697232019 @default.
• W2080469723 countsByYear W20804697232020 @default.
• W2080469723 countsByYear W20804697232021 @default.
• W2080469723 countsByYear W20804697232022 @default.
• W2080469723 countsByYear W20804697232023 @default.
• W2080469723 crossrefType "journal-article" @default.
• W2080469723 hasAuthorship W2080469723A5027995244 @default.
• W2080469723 hasAuthorship W2080469723A5036009102 @default.
• W2080469723 hasAuthorship W2080469723A5071612808 @default.
• W2080469723 hasAuthorship W2080469723A5074001179 @default.
• W2080469723 hasBestOaLocation W20804697231 @default.
• W2080469723 hasConcept C116716535 @default.
• W2080469723 hasConcept C147483822 @default.
• W2080469723 hasConcept C153911025 @default.

• next