Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080470812> ?p ?o ?g. }
- W2080470812 endingPage "1045" @default.
- W2080470812 startingPage "1036" @default.
- W2080470812 abstract "A new method that uses a combination of bacterial GroE chaperonins and cellular osmolytes for in vitro protein folding is described. With this method, one can form stable chaperonin–protein folding intermediate complexes to prevent deleterious protein aggregation and, using these complexes, screen a large array of osmolyte solutions to rapidly identify the superior folding conditions. As a test substrate, we used GSΔ468, a truncation mutant of bacterial glutamine synthetase (GS) that cannot be refolded to significant yields in vitro with either chaperones or osmolytes alone. When our chaperonin/osmolyte method was employed to identify and optimize GSΔ468 refolding conditions, 67% of enzyme activity was recovered, comparable with refolding yields of wild type GS. This method can potentially be applied to the refolding of a broad spectrum of proteins. © 2000 Wiley‐Liss, Inc. and the American Pharmaceutical Association J Pharm Sci 89: 1036–1045, 2000" @default.
- W2080470812 created "2016-06-24" @default.
- W2080470812 creator A5001974475 @default.
- W2080470812 creator A5055232045 @default.
- W2080470812 creator A5083917066 @default.
- W2080470812 date "2000-08-01" @default.
- W2080470812 modified "2023-09-23" @default.
- W2080470812 title "Refolding a Glutamine Synthetase Truncation Mutant In Vitro: Identifying Superior Conditions Using a Combination of Chaperonins and Osmolytes" @default.
- W2080470812 cites W1487327920 @default.
- W2080470812 cites W1503352808 @default.
- W2080470812 cites W1504479686 @default.
- W2080470812 cites W1528013712 @default.
- W2080470812 cites W1531453124 @default.
- W2080470812 cites W1532213170 @default.
- W2080470812 cites W1578896704 @default.
- W2080470812 cites W1580333759 @default.
- W2080470812 cites W1582365370 @default.
- W2080470812 cites W1583226380 @default.
- W2080470812 cites W1608932825 @default.
- W2080470812 cites W1850804182 @default.
- W2080470812 cites W1949018849 @default.
- W2080470812 cites W1970388314 @default.
- W2080470812 cites W1976179026 @default.
- W2080470812 cites W1977245883 @default.
- W2080470812 cites W1977500946 @default.
- W2080470812 cites W1986115510 @default.
- W2080470812 cites W1988259156 @default.
- W2080470812 cites W1990493339 @default.
- W2080470812 cites W1990728291 @default.
- W2080470812 cites W2013973914 @default.
- W2080470812 cites W2014099016 @default.
- W2080470812 cites W2016402887 @default.
- W2080470812 cites W2018096567 @default.
- W2080470812 cites W2023002165 @default.
- W2080470812 cites W2024424860 @default.
- W2080470812 cites W2029651104 @default.
- W2080470812 cites W2040526720 @default.
- W2080470812 cites W2046615922 @default.
- W2080470812 cites W2046872769 @default.
- W2080470812 cites W2049888250 @default.
- W2080470812 cites W2050259519 @default.
- W2080470812 cites W2053514414 @default.
- W2080470812 cites W2055925084 @default.
- W2080470812 cites W2062778876 @default.
- W2080470812 cites W2069141366 @default.
- W2080470812 cites W2082329995 @default.
- W2080470812 cites W2084990004 @default.
- W2080470812 cites W2099861711 @default.
- W2080470812 cites W2100431233 @default.
- W2080470812 cites W2112374350 @default.
- W2080470812 cites W2117934154 @default.
- W2080470812 cites W2123536401 @default.
- W2080470812 cites W2150041704 @default.
- W2080470812 cites W24535451 @default.
- W2080470812 doi "https://doi.org/10.1002/1520-6017(200008)89:8<1036::aid-jps8>3.0.co;2-5" @default.
- W2080470812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10906727" @default.
- W2080470812 hasPublicationYear "2000" @default.
- W2080470812 type Work @default.
- W2080470812 sameAs 2080470812 @default.
- W2080470812 citedByCount "27" @default.
- W2080470812 countsByYear W20804708122013 @default.
- W2080470812 countsByYear W20804708122016 @default.
- W2080470812 countsByYear W20804708122017 @default.
- W2080470812 countsByYear W20804708122018 @default.
- W2080470812 countsByYear W20804708122022 @default.
- W2080470812 crossrefType "journal-article" @default.
- W2080470812 hasAuthorship W2080470812A5001974475 @default.
- W2080470812 hasAuthorship W2080470812A5055232045 @default.
- W2080470812 hasAuthorship W2080470812A5083917066 @default.
- W2080470812 hasConcept C104317684 @default.
- W2080470812 hasConcept C119599485 @default.
- W2080470812 hasConcept C127160389 @default.
- W2080470812 hasConcept C127413603 @default.
- W2080470812 hasConcept C143065580 @default.
- W2080470812 hasConcept C147816474 @default.
- W2080470812 hasConcept C181199279 @default.
- W2080470812 hasConcept C185592680 @default.
- W2080470812 hasConcept C202751555 @default.
- W2080470812 hasConcept C204328495 @default.
- W2080470812 hasConcept C2776545253 @default.
- W2080470812 hasConcept C2779289688 @default.
- W2080470812 hasConcept C2779349466 @default.
- W2080470812 hasConcept C515207424 @default.
- W2080470812 hasConcept C55493867 @default.
- W2080470812 hasConcept C85755625 @default.
- W2080470812 hasConceptScore W2080470812C104317684 @default.
- W2080470812 hasConceptScore W2080470812C119599485 @default.
- W2080470812 hasConceptScore W2080470812C127160389 @default.
- W2080470812 hasConceptScore W2080470812C127413603 @default.
- W2080470812 hasConceptScore W2080470812C143065580 @default.
- W2080470812 hasConceptScore W2080470812C147816474 @default.
- W2080470812 hasConceptScore W2080470812C181199279 @default.
- W2080470812 hasConceptScore W2080470812C185592680 @default.
- W2080470812 hasConceptScore W2080470812C202751555 @default.
- W2080470812 hasConceptScore W2080470812C204328495 @default.
- W2080470812 hasConceptScore W2080470812C2776545253 @default.
- W2080470812 hasConceptScore W2080470812C2779289688 @default.
- W2080470812 hasConceptScore W2080470812C2779349466 @default.