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- W2080471625 abstract "An efficient method for the purification of genomic RNA from the retrovirus, caprine arthritis-encephalitis virus, is described. The method utilizes proteinase K, extraction with sodium perchlorate and chromatography on oligo(dT)-cellulose and results in highly purified RNA capable of being chemically iodinated with Na125 I to high specific radioactivity. The iodinated RNA exhibits 80-90% precipitability in 5% trichloroacetic acid and is greater than or equal to 99% sensitive to hydrolysis by ribonuclease. Several alternative methods which are effective for the preparation of eukaryotic ribosomal RNA are unreliable for purification of retrovirus RNA suitable for radioiodination." @default.
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- W2080471625 date "1982-08-01" @default.
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- W2080471625 title "Purification of retrovirus genomic RNA suitable for chemical radioiodination" @default.
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- W2080471625 doi "https://doi.org/10.1016/0166-0934(82)90056-8" @default.
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