FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080477951> ?p ?o ?g. }

• W2080477951 endingPage "186" @default.
• W2080477951 startingPage "177" @default.
• W2080477951 abstract "Allogeneic donor T lymphocytes manipulated genetically to express the herpes simplex virus thymidine kinase (HSV-TK) gene have emerged as promising tools to alter the balance between graft versus host disease and graft versus leukemia after allogeneic stem cell transplantation, since they can be eliminated selectively in vivo with ganciclovir. Recently, it was reported that in SFCMM-3, an HSV-TK-encoding retroviral vector, two cryptic splice sites in the HSV-TK sequence led to the generation of an HSV-TK splice variant (deltaHSV-TK) that encodes a ganciclovir-resistant gene product. In order to quantify wtHSV-TK and deltaHSV-TK RNA levels we have developed two real time Taqman PCR assays. We demonstrate that the sensitivity of both PCR assays is 10(-4). It was found that the splice variant is generated in the packaging cell line and results in approximately 4.8+/-1.9% of virions that contain deltaHSV-TK RNA. After transduction of human T cells no significant increase in deltaHSV-TK RNA could be detected. Thus, at maximum 4.2+/-1.2% of T cells transduced with SFCMM-3 will be resistant to ganciclovir due to this mechanism only. Together, these assays provide a powerful method to monitor patients in future clinical trials." @default.
• W2080477951 created "2016-06-24" @default.
• W2080477951 creator A5014916171 @default.
• W2080477951 creator A5018148581 @default.
• W2080477951 creator A5031471358 @default.
• W2080477951 creator A5043162057 @default.
• W2080477951 creator A5043913216 @default.
• W2080477951 creator A5074749952 @default.
• W2080477951 creator A5022243346 @default.
• W2080477951 date "2003-05-01" @default.
• W2080477951 modified "2023-09-27" @default.
• W2080477951 title "Development and application of quantitative real time PCR and RT-PCR assays that discriminate between the full-length and truncated herpes simplex virus thymidine kinase gene" @default.
• W2080477951 cites W121638319 @default.
• W2080477951 cites W1479909146 @default.
• W2080477951 cites W1499984377 @default.
• W2080477951 cites W1545991364 @default.
• W2080477951 cites W1707184873 @default.
• W2080477951 cites W1883328448 @default.
• W2080477951 cites W1987746867 @default.
• W2080477951 cites W1991026262 @default.
• W2080477951 cites W1994091239 @default.
• W2080477951 cites W1998609613 @default.
• W2080477951 cites W2021397335 @default.
• W2080477951 cites W2021736875 @default.
• W2080477951 cites W2071024611 @default.
• W2080477951 cites W2076411641 @default.
• W2080477951 cites W2089049593 @default.
• W2080477951 cites W2112041130 @default.
• W2080477951 cites W2131706663 @default.
• W2080477951 cites W2145612365 @default.
• W2080477951 cites W2152291507 @default.
• W2080477951 cites W2160331523 @default.
• W2080477951 cites W2171653261 @default.
• W2080477951 cites W2175611237 @default.
• W2080477951 cites W2180547700 @default.
• W2080477951 cites W2226146977 @default.
• W2080477951 cites W2249621957 @default.
• W2080477951 cites W2330203959 @default.
• W2080477951 cites W2400663726 @default.
• W2080477951 cites W2416148102 @default.
• W2080477951 cites W2418889500 @default.
• W2080477951 cites W2500901422 @default.
• W2080477951 cites W4246085869 @default.
• W2080477951 cites W44995847 @default.
• W2080477951 doi "https://doi.org/10.1016/s0166-0934(03)00069-7" @default.
• W2080477951 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12711061" @default.
• W2080477951 hasPublicationYear "2003" @default.
• W2080477951 type Work @default.
• W2080477951 sameAs 2080477951 @default.
• W2080477951 citedByCount "6" @default.
• W2080477951 countsByYear W20804779512014 @default.
• W2080477951 countsByYear W20804779512015 @default.
• W2080477951 countsByYear W20804779512019 @default.
• W2080477951 countsByYear W20804779512022 @default.
• W2080477951 crossrefType "journal-article" @default.
• W2080477951 hasAuthorship W2080477951A5014916171 @default.
• W2080477951 hasAuthorship W2080477951A5018148581 @default.
• W2080477951 hasAuthorship W2080477951A5022243346 @default.
• W2080477951 hasAuthorship W2080477951A5031471358 @default.
• W2080477951 hasAuthorship W2080477951A5043162057 @default.
• W2080477951 hasAuthorship W2080477951A5043913216 @default.
• W2080477951 hasAuthorship W2080477951A5074749952 @default.
• W2080477951 hasConcept C104317684 @default.
• W2080477951 hasConcept C153911025 @default.
• W2080477951 hasConcept C159047783 @default.
• W2080477951 hasConcept C2522874641 @default.
• W2080477951 hasConcept C2776147627 @default.
• W2080477951 hasConcept C2777965375 @default.
• W2080477951 hasConcept C2779820661 @default.
• W2080477951 hasConcept C2781196997 @default.
• W2080477951 hasConcept C54355233 @default.
• W2080477951 hasConcept C81885089 @default.
• W2080477951 hasConcept C86803240 @default.
• W2080477951 hasConceptScore W2080477951C104317684 @default.
• W2080477951 hasConceptScore W2080477951C153911025 @default.
• W2080477951 hasConceptScore W2080477951C159047783 @default.
• W2080477951 hasConceptScore W2080477951C2522874641 @default.
• W2080477951 hasConceptScore W2080477951C2776147627 @default.
• W2080477951 hasConceptScore W2080477951C2777965375 @default.
• W2080477951 hasConceptScore W2080477951C2779820661 @default.
• W2080477951 hasConceptScore W2080477951C2781196997 @default.
• W2080477951 hasConceptScore W2080477951C54355233 @default.
• W2080477951 hasConceptScore W2080477951C81885089 @default.
• W2080477951 hasConceptScore W2080477951C86803240 @default.
• W2080477951 hasIssue "2" @default.
• W2080477951 hasLocation W20804779511 @default.
• W2080477951 hasLocation W20804779512 @default.
• W2080477951 hasOpenAccess W2080477951 @default.
• W2080477951 hasPrimaryLocation W20804779511 @default.
• W2080477951 hasRelatedWork W1972241043 @default.
• W2080477951 hasRelatedWork W1979990769 @default.
• W2080477951 hasRelatedWork W1994397468 @default.
• W2080477951 hasRelatedWork W2005285469 @default.
• W2080477951 hasRelatedWork W2019754652 @default.
• W2080477951 hasRelatedWork W2044033788 @default.
• W2080477951 hasRelatedWork W2102565620 @default.
• W2080477951 hasRelatedWork W2162468440 @default.
• W2080477951 hasRelatedWork W296738852 @default.

• next