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• W2080480217 abstract "With the increasing use of capillary electrophoresis (CE) in the biotechnology industry, there is a demand for analytical tools and methodology that can be used to characterize CE profiles. This article describes the implementation and optimization of a robust online CE-mass spectrometry (CE-MS) system used for the characterization of several CE assays developed at Genentech Inc. These assays include CE as a complement to reverse-phase peptide mapping for the identification of small peptides eluting in the void volume, profiling N-linked glycopeptide heterogeneity, and determining O-linked site occupancy. In addition, CE-MS was used to confirm major 8-aminopyrene-1,3,6-trisulfonate (APTS)-labeled glycans released from recombinant antibodies that are routinely profiled by CE-laser-induced fluorescence (CE-LIF). For each study, CE-MS was able to successfully identify components seen in UV or LIF electropherograms, thereby expanding the capability of CE and CE-MS for profiling biomolecules." @default.
• W2080480217 created "2016-06-24" @default.
• W2080480217 creator A5061381802 @default.
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• W2080480217 date "2006-08-01" @default.
• W2080480217 modified "2023-10-14" @default.
• W2080480217 title "Capillary electrophoresis–mass spectrometry as a characterization tool for therapeutic proteins" @default.
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• W2080480217 doi "https://doi.org/10.1016/j.ab.2006.04.002" @default.
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