Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080482910> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2080482910 endingPage "98" @default.
- W2080482910 startingPage "94" @default.
- W2080482910 abstract "A highly reactive cysteine residue in class pi glutathione S ‐transferases enhances their susceptibility to chemical alkylation and oxidative stress. Alkylation of the reactive Cys 45 in the porcine class pi enzyme (pGSTP1‐1) with either N ‐iodoacetyl‐ N ′‐(5‐sulpho‐1‐naphthyl)ethylenediamine or iodoacetamide results in a loss of enzyme activity and glutathione‐binding function. Similarly, oxidation of pGSTP1‐1 with hydrogen peroxide (H 2 O 2 ) also results in a loss of catalytic and gluthathione‐binding function, but these effects are reversed by the addition of 5 mM glutathione or dithiothreitol. Analysis by SDS‐PAGE of the H 2 O 2 ‐oxidised enzyme indicates oxidation‐induced formation of disulphide bonds involving Cys 45 . Equilibrium‐unfolding studies with guanidinium chloride indicate that the unfolding of Cys 45 ‐alkylated and H 2 O 2 ‐oxidised pGSTP1‐1 can be described by a two‐state model in which the predominant thermodynamically stable species are the folded dimer and unfolded monomer. Unfolding transition curves suggest that the introduction of a large and bulky AEDANS at Cys 45 does not affect the unfolding pathway for pGSTP1‐1. H 2 O 2 ‐oxidised pGSTP1‐1, on the other hand, appears to follow a different unfolding pathway. This appears not to be a result of the introduction of disulphide bonds since the reduction of these bonds in the oxidised protein with dithiothreitol does not affect the unfolding transition. Furthermore, the conformational stability of the oxidised protein is significantly diminished ( Δ G(H 2 O) = 11.6 kcal/mol) when compared with unmodified and AEDANS‐alkylated enzyme ( Δ G(H 2 O) = 22.5 kcal/mol)." @default.
- W2080482910 created "2016-06-24" @default.
- W2080482910 creator A5041509012 @default.
- W2080482910 creator A5044933366 @default.
- W2080482910 date "1995-09-04" @default.
- W2080482910 modified "2023-10-14" @default.
- W2080482910 title "Conformational stability of Cys<sup>45</sup>-alkylated and hydrogen peroxide-oxidised glutathione<i>S</i>-transferase" @default.
- W2080482910 cites W1505902561 @default.
- W2080482910 cites W1541838625 @default.
- W2080482910 cites W1544541054 @default.
- W2080482910 cites W1563736449 @default.
- W2080482910 cites W1572376186 @default.
- W2080482910 cites W1902072415 @default.
- W2080482910 cites W1913398705 @default.
- W2080482910 cites W1968827252 @default.
- W2080482910 cites W1969419384 @default.
- W2080482910 cites W1979702489 @default.
- W2080482910 cites W1987672033 @default.
- W2080482910 cites W2013324569 @default.
- W2080482910 cites W2018476302 @default.
- W2080482910 cites W2026333941 @default.
- W2080482910 cites W2026474027 @default.
- W2080482910 cites W2033565887 @default.
- W2080482910 cites W2040535337 @default.
- W2080482910 cites W2067709872 @default.
- W2080482910 cites W2072594030 @default.
- W2080482910 cites W2075772525 @default.
- W2080482910 cites W2078057889 @default.
- W2080482910 cites W2079178286 @default.
- W2080482910 cites W2079351920 @default.
- W2080482910 cites W2100837269 @default.
- W2080482910 cites W2235879002 @default.
- W2080482910 cites W337892304 @default.
- W2080482910 doi "https://doi.org/10.1016/0014-5793(95)00826-u" @default.
- W2080482910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7672131" @default.
- W2080482910 hasPublicationYear "1995" @default.
- W2080482910 type Work @default.
- W2080482910 sameAs 2080482910 @default.
- W2080482910 citedByCount "11" @default.
- W2080482910 countsByYear W20804829102013 @default.
- W2080482910 countsByYear W20804829102014 @default.
- W2080482910 crossrefType "journal-article" @default.
- W2080482910 hasAuthorship W2080482910A5041509012 @default.
- W2080482910 hasAuthorship W2080482910A5044933366 @default.
- W2080482910 hasConcept C155647269 @default.
- W2080482910 hasConcept C161790260 @default.
- W2080482910 hasConcept C164361826 @default.
- W2080482910 hasConcept C178790620 @default.
- W2080482910 hasConcept C181199279 @default.
- W2080482910 hasConcept C185592680 @default.
- W2080482910 hasConcept C2776515853 @default.
- W2080482910 hasConcept C2777654166 @default.
- W2080482910 hasConcept C2777824588 @default.
- W2080482910 hasConcept C2779201268 @default.
- W2080482910 hasConcept C2779546866 @default.
- W2080482910 hasConcept C533411734 @default.
- W2080482910 hasConcept C538909803 @default.
- W2080482910 hasConcept C71240020 @default.
- W2080482910 hasConceptScore W2080482910C155647269 @default.
- W2080482910 hasConceptScore W2080482910C161790260 @default.
- W2080482910 hasConceptScore W2080482910C164361826 @default.
- W2080482910 hasConceptScore W2080482910C178790620 @default.
- W2080482910 hasConceptScore W2080482910C181199279 @default.
- W2080482910 hasConceptScore W2080482910C185592680 @default.
- W2080482910 hasConceptScore W2080482910C2776515853 @default.
- W2080482910 hasConceptScore W2080482910C2777654166 @default.
- W2080482910 hasConceptScore W2080482910C2777824588 @default.
- W2080482910 hasConceptScore W2080482910C2779201268 @default.
- W2080482910 hasConceptScore W2080482910C2779546866 @default.
- W2080482910 hasConceptScore W2080482910C533411734 @default.
- W2080482910 hasConceptScore W2080482910C538909803 @default.
- W2080482910 hasConceptScore W2080482910C71240020 @default.
- W2080482910 hasIssue "2" @default.
- W2080482910 hasLocation W20804829101 @default.
- W2080482910 hasLocation W20804829102 @default.
- W2080482910 hasOpenAccess W2080482910 @default.
- W2080482910 hasPrimaryLocation W20804829101 @default.
- W2080482910 hasRelatedWork W1627927351 @default.
- W2080482910 hasRelatedWork W1681688057 @default.
- W2080482910 hasRelatedWork W1981515610 @default.
- W2080482910 hasRelatedWork W2016704697 @default.
- W2080482910 hasRelatedWork W2026532547 @default.
- W2080482910 hasRelatedWork W2028479638 @default.
- W2080482910 hasRelatedWork W2051039670 @default.
- W2080482910 hasRelatedWork W2077235294 @default.
- W2080482910 hasRelatedWork W2254888601 @default.
- W2080482910 hasRelatedWork W2742863538 @default.
- W2080482910 hasVolume "371" @default.
- W2080482910 isParatext "false" @default.
- W2080482910 isRetracted "false" @default.
- W2080482910 magId "2080482910" @default.
- W2080482910 workType "article" @default.