Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080485982> ?p ?o ?g. }
- W2080485982 endingPage "161" @default.
- W2080485982 startingPage "150" @default.
- W2080485982 abstract "The applicability of small interfering RNA (siRNA) in future therapies depends on the availability of safe and efficient carrier systems. Ideally, siRNA delivery requires a system that is stable in the circulation but upon specific uptake into target cells can rapidly release its cargo into the cytoplasm. Previously, we evaluated a novel generation of carrier systems (“decationized” polyplexes) for DNA delivery, and it was shown that folate targeted decationized polyplexes had an excellent safety profile and showed intracellular triggered release upon cell specific uptake. Targeted decationized polyplexes consist of a core of disulfide cross-linked poly(hydroxypropyl methacrylamide) (pHPMA) stably entrapping nucleic acids and a shell of poly(ethylene glycol) (PEG) decorated with folate molecules. In the present study, the applicability of folate targeted decationized polyplexes for siRNA delivery was investigated. This required optimization of the carrier system particularly regarding the cross-linking density of the core of the polyplexes. Stable and nanosized siRNA decationized polyplexes were successfully prepared by optimizing the cross-link density of their core. Upon incubation in human plasma, a significant portion of siRNA remained entrapped in the decationized polyplexes as determined by fluorescence correlation spectroscopy (FCS). When tested in a folate receptor overexpressing cell line stably expressing luciferase, Skov3-luc, sequence specific gene silencing was observed. As expected, neither interference on the intrinsic luciferase expression nor on the cell metabolic activity (determined by XTT) was induced by the free-polymer or the siRNA polyplexes. In conclusion, targeted decationized polyplexes are safe and stable carriers that interact with the targeted cells and rapidly disassemble upon cell entry making them promising siRNA delivery systems." @default.
- W2080485982 created "2016-06-24" @default.
- W2080485982 creator A5017735982 @default.
- W2080485982 creator A5026683403 @default.
- W2080485982 creator A5028407212 @default.
- W2080485982 creator A5036902171 @default.
- W2080485982 creator A5040705718 @default.
- W2080485982 creator A5041253854 @default.
- W2080485982 creator A5051906887 @default.
- W2080485982 creator A5069511584 @default.
- W2080485982 creator A5072262909 @default.
- W2080485982 creator A5073457405 @default.
- W2080485982 date "2014-11-20" @default.
- W2080485982 modified "2023-09-30" @default.
- W2080485982 title "Targeted Decationized Polyplexes for siRNA Delivery" @default.
- W2080485982 cites W1963973175 @default.
- W2080485982 cites W1964146045 @default.
- W2080485982 cites W1965393657 @default.
- W2080485982 cites W1972545546 @default.
- W2080485982 cites W1979389654 @default.
- W2080485982 cites W1984811676 @default.
- W2080485982 cites W1985088153 @default.
- W2080485982 cites W1991716060 @default.
- W2080485982 cites W1995997091 @default.
- W2080485982 cites W1998697301 @default.
- W2080485982 cites W2000136129 @default.
- W2080485982 cites W2002351185 @default.
- W2080485982 cites W2003280484 @default.
- W2080485982 cites W2003908748 @default.
- W2080485982 cites W2007086477 @default.
- W2080485982 cites W2007347651 @default.
- W2080485982 cites W2011685239 @default.
- W2080485982 cites W2012395572 @default.
- W2080485982 cites W2015325415 @default.
- W2080485982 cites W2015585578 @default.
- W2080485982 cites W2015917934 @default.
- W2080485982 cites W2022564018 @default.
- W2080485982 cites W2024939468 @default.
- W2080485982 cites W2028055406 @default.
- W2080485982 cites W2028973041 @default.
- W2080485982 cites W2029289994 @default.
- W2080485982 cites W2030855909 @default.
- W2080485982 cites W2032093175 @default.
- W2080485982 cites W2036474910 @default.
- W2080485982 cites W2043831637 @default.
- W2080485982 cites W2046210384 @default.
- W2080485982 cites W2047609968 @default.
- W2080485982 cites W2050886209 @default.
- W2080485982 cites W2055351480 @default.
- W2080485982 cites W2056311063 @default.
- W2080485982 cites W2058506859 @default.
- W2080485982 cites W2060158020 @default.
- W2080485982 cites W2060882819 @default.
- W2080485982 cites W2067302150 @default.
- W2080485982 cites W2074502352 @default.
- W2080485982 cites W2074870430 @default.
- W2080485982 cites W2078154125 @default.
- W2080485982 cites W2078377919 @default.
- W2080485982 cites W2079933337 @default.
- W2080485982 cites W2086069830 @default.
- W2080485982 cites W2089645494 @default.
- W2080485982 cites W2093713807 @default.
- W2080485982 cites W2094422309 @default.
- W2080485982 cites W2103719856 @default.
- W2080485982 cites W2104050632 @default.
- W2080485982 cites W2108882170 @default.
- W2080485982 cites W2120486710 @default.
- W2080485982 cites W2121171143 @default.
- W2080485982 cites W2122140226 @default.
- W2080485982 cites W2123571664 @default.
- W2080485982 cites W2131083445 @default.
- W2080485982 cites W2133276513 @default.
- W2080485982 cites W2134796795 @default.
- W2080485982 cites W2140595006 @default.
- W2080485982 cites W2143208806 @default.
- W2080485982 cites W2166284356 @default.
- W2080485982 cites W2313466390 @default.
- W2080485982 cites W2320450560 @default.
- W2080485982 cites W2324233275 @default.
- W2080485982 cites W4211039187 @default.
- W2080485982 cites W4244618912 @default.
- W2080485982 cites W68151451 @default.
- W2080485982 doi "https://doi.org/10.1021/mp500499x" @default.
- W2080485982 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25384057" @default.
- W2080485982 hasPublicationYear "2014" @default.
- W2080485982 type Work @default.
- W2080485982 sameAs 2080485982 @default.
- W2080485982 citedByCount "21" @default.
- W2080485982 countsByYear W20804859822014 @default.
- W2080485982 countsByYear W20804859822015 @default.
- W2080485982 countsByYear W20804859822016 @default.
- W2080485982 countsByYear W20804859822017 @default.
- W2080485982 countsByYear W20804859822018 @default.
- W2080485982 countsByYear W20804859822019 @default.
- W2080485982 countsByYear W20804859822020 @default.
- W2080485982 countsByYear W20804859822021 @default.
- W2080485982 crossrefType "journal-article" @default.
- W2080485982 hasAuthorship W2080485982A5017735982 @default.