FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080489511> ?p ?o ?g. }

• W2080489511 endingPage "27568" @default.
• W2080489511 startingPage "27562" @default.
• W2080489511 abstract "Fluorescence resonance energy transfer (FRET) was used to investigate whether interleukin-1 (IL-1) causes the aggregation of IL-1 type I receptors (IL-1 RI) at the cell surface. For these experiments, a noncompetitive anti-IL1 RI monoclonal antibody, M5, was labeled separately with a donor probe, fluorescein isothiocyanate, or with an acceptor carbocyanine probe, Cy3. Donor-labeled M5 and acceptor-labeled M5 were simultaneously bound to transfected mouse IL-1 RI on either C-127 mouse mammary carcinoma cells or on Chinese hamster ovary (CHO)-K1 cells, and the ratio of acceptor emission at 590 nm to donor emission at 525 nm (excitation at 488 and 514 nm) was monitored with flow cytometry as an indicator of FRET. Addition of a saturating concentration of human IL-1α at 22°C causes a time-dependent increase in FRET for both cell lines that indicates IL-1-dependent self-association of IL-1 RI. Binding of the IL-1 receptor antagonist at 22°C causes little or no FRET for both cell lines, indicating a correlation between receptor aggregation and the ability of the ligand to stimulate a functional response. When donor-labeled and acceptor-labeled Fab fragments of M5 are used to monitor FRET, IL-1α causes efficient energy transfer in the CHO-K1 cells at 22°C, but not at 4°C. In contrast, IL-1α causes much less FRET at 22°C in C-127 cells when the M5 Fab fragments are used instead of the intact bivalent M5. In a striking parallel, IL-1α-dependent activation of prostaglandin E2 production depends on the bivalent M5 antibody in the C-127 cells, but is independent of this monoclonal antibody in the CHO-K1 cells. These results provide a strong correlation between the ability of IL-1 to cause the aggregation of IL-1 RI and the stimulation of a functional response. Fluorescence resonance energy transfer (FRET) was used to investigate whether interleukin-1 (IL-1) causes the aggregation of IL-1 type I receptors (IL-1 RI) at the cell surface. For these experiments, a noncompetitive anti-IL1 RI monoclonal antibody, M5, was labeled separately with a donor probe, fluorescein isothiocyanate, or with an acceptor carbocyanine probe, Cy3. Donor-labeled M5 and acceptor-labeled M5 were simultaneously bound to transfected mouse IL-1 RI on either C-127 mouse mammary carcinoma cells or on Chinese hamster ovary (CHO)-K1 cells, and the ratio of acceptor emission at 590 nm to donor emission at 525 nm (excitation at 488 and 514 nm) was monitored with flow cytometry as an indicator of FRET. Addition of a saturating concentration of human IL-1α at 22°C causes a time-dependent increase in FRET for both cell lines that indicates IL-1-dependent self-association of IL-1 RI. Binding of the IL-1 receptor antagonist at 22°C causes little or no FRET for both cell lines, indicating a correlation between receptor aggregation and the ability of the ligand to stimulate a functional response. When donor-labeled and acceptor-labeled Fab fragments of M5 are used to monitor FRET, IL-1α causes efficient energy transfer in the CHO-K1 cells at 22°C, but not at 4°C. In contrast, IL-1α causes much less FRET at 22°C in C-127 cells when the M5 Fab fragments are used instead of the intact bivalent M5. In a striking parallel, IL-1α-dependent activation of prostaglandin E2 production depends on the bivalent M5 antibody in the C-127 cells, but is independent of this monoclonal antibody in the CHO-K1 cells. These results provide a strong correlation between the ability of IL-1 to cause the aggregation of IL-1 RI and the stimulation of a functional response." @default.
• W2080489511 created "2016-06-24" @default.
• W2080489511 creator A5002939449 @default.
• W2080489511 creator A5009580916 @default.
• W2080489511 creator A5048526342 @default.
• W2080489511 creator A5067796950 @default.
• W2080489511 date "1995-11-01" @default.
• W2080489511 modified "2023-09-27" @default.
• W2080489511 title "Fluorescence Resonance Energy Transfer Reveals Interleukin (IL)-1-dependent Aggregation of IL-1 Type I Receptors That Correlates with Receptor Activation" @default.
• W2080489511 cites W1487282140 @default.
• W2080489511 cites W1514081838 @default.
• W2080489511 cites W1521031916 @default.
• W2080489511 cites W1543377758 @default.
• W2080489511 cites W1548933760 @default.
• W2080489511 cites W1565066731 @default.
• W2080489511 cites W1574330564 @default.
• W2080489511 cites W1586009176 @default.
• W2080489511 cites W1640758599 @default.
• W2080489511 cites W1973766879 @default.
• W2080489511 cites W1975331230 @default.
• W2080489511 cites W1982932317 @default.
• W2080489511 cites W1989392905 @default.
• W2080489511 cites W2007210793 @default.
• W2080489511 cites W2011253952 @default.
• W2080489511 cites W2018136510 @default.
• W2080489511 cites W2029803796 @default.
• W2080489511 cites W2032359283 @default.
• W2080489511 cites W2036895735 @default.
• W2080489511 cites W2054154416 @default.
• W2080489511 cites W2055131745 @default.
• W2080489511 cites W2061896427 @default.
• W2080489511 cites W2067478797 @default.
• W2080489511 cites W2068797099 @default.
• W2080489511 cites W2073977304 @default.
• W2080489511 cites W2075125834 @default.
• W2080489511 cites W2078136106 @default.
• W2080489511 cites W2123730940 @default.
• W2080489511 cites W2125499492 @default.
• W2080489511 cites W2128830755 @default.
• W2080489511 cites W2199620244 @default.
• W2080489511 cites W22124861 @default.
• W2080489511 cites W237143463 @default.
• W2080489511 cites W2395259494 @default.
• W2080489511 cites W645989409 @default.
• W2080489511 doi "https://doi.org/10.1074/jbc.270.46.27562" @default.
• W2080489511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7499217" @default.
• W2080489511 hasPublicationYear "1995" @default.
• W2080489511 type Work @default.
• W2080489511 sameAs 2080489511 @default.
• W2080489511 citedByCount "52" @default.
• W2080489511 countsByYear W20804895112015 @default.
• W2080489511 countsByYear W20804895112017 @default.
• W2080489511 countsByYear W20804895112019 @default.
• W2080489511 crossrefType "journal-article" @default.
• W2080489511 hasAuthorship W2080489511A5002939449 @default.
• W2080489511 hasAuthorship W2080489511A5009580916 @default.
• W2080489511 hasAuthorship W2080489511A5048526342 @default.
• W2080489511 hasAuthorship W2080489511A5067796950 @default.
• W2080489511 hasBestOaLocation W20804895111 @default.
• W2080489511 hasConcept C121332964 @default.
• W2080489511 hasConcept C12554922 @default.
• W2080489511 hasConcept C153911025 @default.
• W2080489511 hasConcept C170493617 @default.
• W2080489511 hasConcept C175656101 @default.
• W2080489511 hasConcept C185592680 @default.
• W2080489511 hasConcept C26873012 @default.
• W2080489511 hasConcept C2777019933 @default.
• W2080489511 hasConcept C2778015030 @default.
• W2080489511 hasConcept C2779892579 @default.
• W2080489511 hasConcept C553184892 @default.
• W2080489511 hasConcept C55493867 @default.
• W2080489511 hasConcept C62520636 @default.
• W2080489511 hasConcept C86803240 @default.
• W2080489511 hasConcept C91881484 @default.
• W2080489511 hasConcept C96305047 @default.
• W2080489511 hasConceptScore W2080489511C121332964 @default.
• W2080489511 hasConceptScore W2080489511C12554922 @default.
• W2080489511 hasConceptScore W2080489511C153911025 @default.
• W2080489511 hasConceptScore W2080489511C170493617 @default.
• W2080489511 hasConceptScore W2080489511C175656101 @default.
• W2080489511 hasConceptScore W2080489511C185592680 @default.
• W2080489511 hasConceptScore W2080489511C26873012 @default.
• W2080489511 hasConceptScore W2080489511C2777019933 @default.
• W2080489511 hasConceptScore W2080489511C2778015030 @default.
• W2080489511 hasConceptScore W2080489511C2779892579 @default.
• W2080489511 hasConceptScore W2080489511C553184892 @default.
• W2080489511 hasConceptScore W2080489511C55493867 @default.
• W2080489511 hasConceptScore W2080489511C62520636 @default.
• W2080489511 hasConceptScore W2080489511C86803240 @default.
• W2080489511 hasConceptScore W2080489511C91881484 @default.
• W2080489511 hasConceptScore W2080489511C96305047 @default.
• W2080489511 hasIssue "46" @default.
• W2080489511 hasLocation W20804895111 @default.
• W2080489511 hasOpenAccess W2080489511 @default.
• W2080489511 hasPrimaryLocation W20804895111 @default.
• W2080489511 hasRelatedWork W1599075286 @default.
• W2080489511 hasRelatedWork W1967874844 @default.
• W2080489511 hasRelatedWork W2017883949 @default.

• next