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- W2080490218 abstract "In a recent communication to the Royal Society, Dr. Bastian brought forward some experiments to show that while an acid urine usually remains barren after being boiled a few minutes, the same urine becomes fertile when similarly treated if previously neutralized or rendered alkaline by liquor potassæ, especially if it be afterwards maintained at a temperature of 115° F. or 122° F. In this respect urine only conforms to the general rule observed by myself and formulated in my previous communication to the Society—that “slightly alkaline liquids were always more difficult to sterilize (by heat) than slightly acid liquids.” This difference came out strongest in my own experiments in the case of hay-infusion—the acid infusion invariably remaining barren after a few minutes boiling, and the neutralized infusion invariably becoming fertile after a similar boiling. Accordingly I utilized hay-infusion to determine the cause of the difference in question. It could evidently only be due to one of two things—either (1) the change of reaction enabled germs preexisting in the infusion to survive the ebullition, or (2) the addition of the alkali exercised a positive influence in exciting a de novo generation of organisms. To decide which of these two interpretations was the true one, an experiment was contrived in which the liq. potassæ could be added to the infusion not before, but after it had been boiled, and thereby rendered permanently sterile. When added in this way, I found that liquor potassæ had not any power to excite germination. The infusions invariably remained barren when the alkali was added to them after they had been sterilized. I therefore concluded that the effect of the change of reaction consisted simply in enabling preexisting germs to survive a brief ebullition. Dr. Bastian, in repeating this experiment in the case of urine, arrived at an opposite conclusion: he found that whether the alkali was added before or after ebullition he obtained the same result—the urine in both cases became fertile; and he concluded that the alkali had a positive power of promoting the origin of organisms in the urine." @default.
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- W2080490218 date "1877-12-31" @default.
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- W2080490218 title "III. Note on the influence of liquor potassæ and an elevated temperature on the origin and growth of microphytes" @default.
- W2080490218 doi "https://doi.org/10.1098/rspl.1876.0067" @default.
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