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• W2080497450 abstract "Permanent pasture stands in northern Victoria (Australia) were used for protein extraction in both laboratory-scale and plant-scale operations during the spring, summer and autumn seasons. Mixed herbage was in its third, fourth, fifth and sixth weeks of regrowth. Efficiency of the protein extraction process at different stages of herbage maturity was measured, as well as the proximate analysis, amino acid composition, availability of lysine, methionine, tryptophan and protein efficiency ratio (PER) of the protein, recovered by both steam coagulation (85 d̀C) and ultrafiltration procedures. Recovery of protein nitrogen from pasture herbage determined by the steam precipitation (85 d̀C) procedure was on average, slightly higher in spring and autumn, as compared with summer. Significant interactions between the seasons and herbage regrowth stages were observed in both herbage dry matter (DM) and crude protein (N × 6.25) content, protein recovery and yield of protein from the unit of herbage DM. In general however, protein recovery declined with herbage maturity. Significantly less leaf protein concentrate (LPC) with lower nitrogen content was produced from yields of herbage in the summer season as compared with that harvested and processed in the spring and autumn seasons. LPC produced using an ultrafiltration procedure was superior to LPC obtained by the steam coagulation process in terms of LPC yield and protein nitrogen recovery, crude protein and total essential amino acids, lysine, threonine and tryptophan content and tryptophan availability. After synthetic DL-methionine and L-lysine supplementations, freeze-dried ultrafiltered LPC gave (in tests with rats) a similar PER to casein which was used as a standard control protein. To achieve a PER value similar to casein, LPC produced by heat coagulation had to be supplemented with L-tryptophan in addition to L-lysine and DL-methionine." @default.
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• W2080497450 date "1980-02-01" @default.
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• W2080497450 title "Quantities and quality of protein extracted from pasture herbage using heat precipitation or ultrafiltration procedures" @default.
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• W2080497450 doi "https://doi.org/10.1002/jsfa.2740310209" @default.
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