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- W2080498475 abstract "DNA hairpins are models for more complex, naturally occurring loop-containing nucleic acid structures. They consist of two distinct structural domains: a double stranded stem and a single-stranded loop that connect the two strands of the stem. Previous studies of short DNA hairpins have revealed that the base sequence of the loop and stem significantly affect the relative thermodynamic stability of the hairpin and coil conformations. We have studied the effect of hydrostatic pressure on the heat-induced helix-coil transition of short DNA hairpins in order to gain knowledge about the role of hydration in the stability of the secondary structure adopted by these self-complementary DNA molecules. We report the effect of hydrostatic pressure on the helix-coil transition temperature (TM), monitored by absorption, for eleven 16-base DNA hairpins at different sodium chloride concentrations. The studied hairpins form by intramolecular folding of 16-base self-complementary DNA oligodeoxyribonucleotides. Each of the hairpins structures has a six-base pair duplex-forming stem linked by a four-base loop. We have varied only loop sequence and identity of the duplex base pair adjacent to the loop; the first four base pairs in the stem are the same for every molecule. Transition parameters based on a two-state denaturation process, such as ΔHvH and transition volume (ΔV), were calculated from the optical melting transitions. Experiments revealed the ΔV for denaturation of these molecules range from −2.35 to +6.74 cm3mol−1. We propose that the sequence dependence of the molar volume changes is attributable to differences in the hydration of these molecules." @default.
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- W2080498475 date "2011-02-01" @default.
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- W2080498475 title "Effect of Pressure on the Stability of Short DNA Hairpins" @default.
- W2080498475 doi "https://doi.org/10.1016/j.bpj.2010.12.2139" @default.
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