Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080508912> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2080508912 endingPage "2077" @default.
- W2080508912 startingPage "2069" @default.
- W2080508912 abstract "Retroviral transduction of antifolate-resistant variants of human dihydrofolate reductase (hDHFR) into cells can increase their resistance to the cytotoxic effects of these drugs. We evaluated the ability of wild-type hDHFR and 20 mutant enzymes (13 with single-amino acid substitutions, 7 with two substitutions) to prevent growth inhibition in antifolate-treated CCRF-CEM cells. The wild-type enzyme and all of the variants significantly protected transduced cells from trimetrexate (TMTX)-induced growth inhibition. However, only half of the variants conferred more protection than does the wild-type enzyme. For the variants tested, the observed protective effect was higher for TMTX than for methotrexate (< or =7.5-fold increased resistance), piritrexim (< or =16-fold), and edatrexate (negligible). Transduction of the variants L22Y-F31S and L22Y-F31R led to the greatest protection against TMTX (approximately 200-fold). Protection from loss of cell viability was similar to protection from growth inhibition. The protection associated with a particular mutant hDHFR did not result from the level of expression: Efficient protection resulted from low affinity of the variant for antifolates, reasonable catalytic activity, and good thermal stability. Clones isolated from a polyclonal population of transduced cells varied by as much as 30-fold in their resistance to TMTX, the resistance differences depending on hDHFR expression levels." @default.
- W2080508912 created "2016-06-24" @default.
- W2080508912 creator A5005945671 @default.
- W2080508912 creator A5012171136 @default.
- W2080508912 creator A5034981457 @default.
- W2080508912 creator A5078253937 @default.
- W2080508912 creator A5082948957 @default.
- W2080508912 creator A5086198464 @default.
- W2080508912 creator A5086845095 @default.
- W2080508912 date "1997-11-20" @default.
- W2080508912 modified "2023-10-10" @default.
- W2080508912 title "Comparison of the Protection of Cells from Antifolates by Transduced Human Dihydrofolate Reductase Mutants" @default.
- W2080508912 cites W1481921317 @default.
- W2080508912 cites W1484504127 @default.
- W2080508912 cites W1527359370 @default.
- W2080508912 cites W1537778845 @default.
- W2080508912 cites W1576579253 @default.
- W2080508912 cites W1594621311 @default.
- W2080508912 cites W1963863085 @default.
- W2080508912 cites W1981209502 @default.
- W2080508912 cites W1984219340 @default.
- W2080508912 cites W1984367762 @default.
- W2080508912 cites W2015581930 @default.
- W2080508912 cites W2024386750 @default.
- W2080508912 cites W2037585283 @default.
- W2080508912 cites W2047861739 @default.
- W2080508912 cites W2064217264 @default.
- W2080508912 cites W2078864554 @default.
- W2080508912 cites W2080501775 @default.
- W2080508912 cites W2093983183 @default.
- W2080508912 cites W2123509576 @default.
- W2080508912 cites W2149795002 @default.
- W2080508912 cites W2401851728 @default.
- W2080508912 cites W2422711059 @default.
- W2080508912 cites W4254714437 @default.
- W2080508912 cites W53701705 @default.
- W2080508912 doi "https://doi.org/10.1089/hum.1997.8.17-2069" @default.
- W2080508912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9414255" @default.
- W2080508912 hasPublicationYear "1997" @default.
- W2080508912 type Work @default.
- W2080508912 sameAs 2080508912 @default.
- W2080508912 citedByCount "28" @default.
- W2080508912 countsByYear W20805089122016 @default.
- W2080508912 countsByYear W20805089122020 @default.
- W2080508912 crossrefType "journal-article" @default.
- W2080508912 hasAuthorship W2080508912A5005945671 @default.
- W2080508912 hasAuthorship W2080508912A5012171136 @default.
- W2080508912 hasAuthorship W2080508912A5034981457 @default.
- W2080508912 hasAuthorship W2080508912A5078253937 @default.
- W2080508912 hasAuthorship W2080508912A5082948957 @default.
- W2080508912 hasAuthorship W2080508912A5086198464 @default.
- W2080508912 hasAuthorship W2080508912A5086845095 @default.
- W2080508912 hasConcept C104317684 @default.
- W2080508912 hasConcept C143065580 @default.
- W2080508912 hasConcept C153911025 @default.
- W2080508912 hasConcept C181199279 @default.
- W2080508912 hasConcept C203014093 @default.
- W2080508912 hasConcept C207583985 @default.
- W2080508912 hasConcept C2778397455 @default.
- W2080508912 hasConcept C2781059491 @default.
- W2080508912 hasConcept C2781320022 @default.
- W2080508912 hasConcept C2781382166 @default.
- W2080508912 hasConcept C55493867 @default.
- W2080508912 hasConcept C86803240 @default.
- W2080508912 hasConcept C98274493 @default.
- W2080508912 hasConceptScore W2080508912C104317684 @default.
- W2080508912 hasConceptScore W2080508912C143065580 @default.
- W2080508912 hasConceptScore W2080508912C153911025 @default.
- W2080508912 hasConceptScore W2080508912C181199279 @default.
- W2080508912 hasConceptScore W2080508912C203014093 @default.
- W2080508912 hasConceptScore W2080508912C207583985 @default.
- W2080508912 hasConceptScore W2080508912C2778397455 @default.
- W2080508912 hasConceptScore W2080508912C2781059491 @default.
- W2080508912 hasConceptScore W2080508912C2781320022 @default.
- W2080508912 hasConceptScore W2080508912C2781382166 @default.
- W2080508912 hasConceptScore W2080508912C55493867 @default.
- W2080508912 hasConceptScore W2080508912C86803240 @default.
- W2080508912 hasConceptScore W2080508912C98274493 @default.
- W2080508912 hasIssue "17" @default.
- W2080508912 hasLocation W20805089121 @default.
- W2080508912 hasLocation W20805089122 @default.
- W2080508912 hasOpenAccess W2080508912 @default.
- W2080508912 hasPrimaryLocation W20805089121 @default.
- W2080508912 hasRelatedWork W1979040389 @default.
- W2080508912 hasRelatedWork W1993532321 @default.
- W2080508912 hasRelatedWork W2017754958 @default.
- W2080508912 hasRelatedWork W2070867384 @default.
- W2080508912 hasRelatedWork W2103288727 @default.
- W2080508912 hasRelatedWork W2137570846 @default.
- W2080508912 hasRelatedWork W2158292139 @default.
- W2080508912 hasRelatedWork W2170816730 @default.
- W2080508912 hasRelatedWork W3807580 @default.
- W2080508912 hasRelatedWork W4220706000 @default.
- W2080508912 hasVolume "8" @default.
- W2080508912 isParatext "false" @default.
- W2080508912 isRetracted "false" @default.
- W2080508912 magId "2080508912" @default.
- W2080508912 workType "article" @default.