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• W2080543462 abstract "Photodynamic therapy (PDT) is a promising method for localized and specific inactivation of fungi and bacteria. A nontoxic light-sensitive compound is taken up by cells, which are then exposed selectively to light, which activates toxicity of the compound. We investigated the potential of sublethal PDT using light-sensitive curcumin (CUR) in combination with blue (455 nm) light to promote reactive oxygen species (ROS) formation in the form of singlet oxygen and DNA damage of Candida albicans. Surprisingly, CUR-mediated PDT but also light alone caused significantly longer comet tails, an indication of DNA damage of C. albicans when compared with the negative control. The intracellular ROS production was also significantly higher for the group treated only with light. However, PDT compared to blue light alone significantly slowed DNA repair. Comet tails decreased during 30 min visualized as a 90% reduction in length in the absence of light for cells treated with light alone, while comet tails of cells treated with PDT only diminished in size about 45%. These results indicate that complex mechanisms may result in PDT in a way that should be considered when choosing the photosensitive compound and other aspects of the treatment design." @default.
• W2080543462 created "2016-06-24" @default.
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• W2080543462 date "2015-04-21" @default.
• W2080543462 modified "2023-10-10" @default.
• W2080543462 title "Genotoxic effect of photodynamic therapy mediated by curcumin on Candida albicans" @default.
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• W2080543462 doi "https://doi.org/10.1093/femsyr/fov018" @default.
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• W2080543462 hasPublicationYear "2015" @default.
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