Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080554706> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2080554706 endingPage "36" @default.
- W2080554706 startingPage "31" @default.
- W2080554706 abstract "The RAD14 gene of Saccharomyces cerevisiae is required for the incision step of the nucleotide excision repair process. The Rad14 protein can bind zinc, possesses a potential zinc finger DNA binding domain and has been shown to bind specifically to damaged DNA. Differences in UV sensitivity exist between a rad14 deletion strain and a putative rad14 point mutant, the point mutant being more resistant to UV than the deletion strain. Here, we confirm that the rad14 deletion strain repairs neither UV-induced cyclobutane pyrimidine dimers (CPDs) nor endonuclease III-sensitive damage sites, whereas the point mutant cannot repair the former but can repair the latter. From this it can be inferred that the point mutant produces an altered protein product allowing recognition of endonuclease III sensitive sites but not CPDs. To investigate this, the rad14 mutant allele was sequenced. It contained two GC-AT transition mutations when compared to the wild-type RAD14 gene sequence. When the rad14 point mutant sequence is translated, alterations within the putative zinc finger binding domain are observed, with one of the cysteine residues of the zinc binding motif being replaced by tyrosine. This suggests that alterations within the zinc finger binding domain of the Rad14 protein cause changes to the damage recognition properties of the protein. The use of the Rad14 protein from the point mutant should assist in experiments investigating the in vitro binding properties of the Rad14 protein to different types of DNA damage." @default.
- W2080554706 created "2016-06-24" @default.
- W2080554706 creator A5052358177 @default.
- W2080554706 creator A5081987606 @default.
- W2080554706 creator A5083595167 @default.
- W2080554706 date "1997-01-01" @default.
- W2080554706 modified "2023-09-25" @default.
- W2080554706 title "Characterization of therad14-2 Mutant ofSaccharomyces cerevisiae: Implications for the Recognition of UV Photoproducts by the Rad14 Protein" @default.
- W2080554706 cites W1612116987 @default.
- W2080554706 cites W1963868441 @default.
- W2080554706 cites W1971134263 @default.
- W2080554706 cites W1984353960 @default.
- W2080554706 cites W1988733863 @default.
- W2080554706 cites W2000936373 @default.
- W2080554706 cites W2009310436 @default.
- W2080554706 cites W2031867299 @default.
- W2080554706 cites W2039068784 @default.
- W2080554706 cites W2054212919 @default.
- W2080554706 cites W2066040575 @default.
- W2080554706 cites W2080151116 @default.
- W2080554706 cites W2089712104 @default.
- W2080554706 cites W2102326894 @default.
- W2080554706 cites W2126830955 @default.
- W2080554706 cites W2150072036 @default.
- W2080554706 doi "https://doi.org/10.1002/(sici)1097-0061(199701)13:1<31::aid-yea60>3.0.co;2-4" @default.
- W2080554706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9046084" @default.
- W2080554706 hasPublicationYear "1997" @default.
- W2080554706 type Work @default.
- W2080554706 sameAs 2080554706 @default.
- W2080554706 citedByCount "2" @default.
- W2080554706 countsByYear W20805547062013 @default.
- W2080554706 crossrefType "journal-article" @default.
- W2080554706 hasAuthorship W2080554706A5052358177 @default.
- W2080554706 hasAuthorship W2080554706A5081987606 @default.
- W2080554706 hasAuthorship W2080554706A5083595167 @default.
- W2080554706 hasConcept C104317684 @default.
- W2080554706 hasConcept C104451858 @default.
- W2080554706 hasConcept C134935766 @default.
- W2080554706 hasConcept C143065580 @default.
- W2080554706 hasConcept C153911025 @default.
- W2080554706 hasConcept C167975912 @default.
- W2080554706 hasConcept C176944494 @default.
- W2080554706 hasConcept C24284526 @default.
- W2080554706 hasConcept C2777028655 @default.
- W2080554706 hasConcept C2777576037 @default.
- W2080554706 hasConcept C2779926675 @default.
- W2080554706 hasConcept C35802184 @default.
- W2080554706 hasConcept C552990157 @default.
- W2080554706 hasConcept C55493867 @default.
- W2080554706 hasConcept C86339819 @default.
- W2080554706 hasConcept C86803240 @default.
- W2080554706 hasConceptScore W2080554706C104317684 @default.
- W2080554706 hasConceptScore W2080554706C104451858 @default.
- W2080554706 hasConceptScore W2080554706C134935766 @default.
- W2080554706 hasConceptScore W2080554706C143065580 @default.
- W2080554706 hasConceptScore W2080554706C153911025 @default.
- W2080554706 hasConceptScore W2080554706C167975912 @default.
- W2080554706 hasConceptScore W2080554706C176944494 @default.
- W2080554706 hasConceptScore W2080554706C24284526 @default.
- W2080554706 hasConceptScore W2080554706C2777028655 @default.
- W2080554706 hasConceptScore W2080554706C2777576037 @default.
- W2080554706 hasConceptScore W2080554706C2779926675 @default.
- W2080554706 hasConceptScore W2080554706C35802184 @default.
- W2080554706 hasConceptScore W2080554706C552990157 @default.
- W2080554706 hasConceptScore W2080554706C55493867 @default.
- W2080554706 hasConceptScore W2080554706C86339819 @default.
- W2080554706 hasConceptScore W2080554706C86803240 @default.
- W2080554706 hasIssue "1" @default.
- W2080554706 hasLocation W20805547061 @default.
- W2080554706 hasLocation W20805547062 @default.
- W2080554706 hasOpenAccess W2080554706 @default.
- W2080554706 hasPrimaryLocation W20805547061 @default.
- W2080554706 hasRelatedWork W1713339284 @default.
- W2080554706 hasRelatedWork W1865915409 @default.
- W2080554706 hasRelatedWork W1963673525 @default.
- W2080554706 hasRelatedWork W1967287923 @default.
- W2080554706 hasRelatedWork W2014892069 @default.
- W2080554706 hasRelatedWork W2080554706 @default.
- W2080554706 hasRelatedWork W2090821575 @default.
- W2080554706 hasRelatedWork W2131176301 @default.
- W2080554706 hasRelatedWork W2156309021 @default.
- W2080554706 hasRelatedWork W4236271576 @default.
- W2080554706 hasVolume "13" @default.
- W2080554706 isParatext "false" @default.
- W2080554706 isRetracted "false" @default.
- W2080554706 magId "2080554706" @default.
- W2080554706 workType "article" @default.