FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080554920> ?p ?o ?g. }

• W2080554920 endingPage "407" @default.
• W2080554920 startingPage "396" @default.
• W2080554920 abstract "Abstract Objectives Although clinical applications using mesenchymal stromal cells ( MSC s) are becoming more frequent, procedures for their in vitro culture are far from standardized. Growth factors such as FGF ‐2 are frequently added during expansion to improve population growth and differentiation characteristics. However, up to now its influence on surface marker distribution of MSC s has been close to unknown. The purpose of this study was therefore to analyse effects of FGF ‐2 supplementation on pre‐selection of MSC subpopulations. Materials and methods Mesenchymal stromal cells were harvested from bone marrow of six patients and expanded in alpha‐ MEM or DMEM ‐ LG . Starting in passage 2, 10 ng/ml FGF ‐2 was administered and non‐supplemented media were used as controls. Growth indices were calculated from P0 to P4. After P4, fluorescence cytometry for common MSC surface markers was performed and standard chondrogenic, adipogenic and osteogenic differentiation protocols were applied. Results Cell population growth indices were higher for those in FGF ‐2 supplemented media. Significant differences in surface marker distribution were observed for CD13, CD14, CD49, CD90, CD340 and STRO‐1 depending on respective culture conditions. FGF ‐2 suppressed CD146 expression in both alpha‐ MEM and DMEM ‐ LG . No differences in adipogenic and osteogenic differentiation potential could be observed, while FGF ‐2 significantly improved chondrogenic differentiation in DMEM ‐ LG . Conclusions While holding the benefit of improving MSC chondrogenic differentiation potential, FGF ‐2 pre‐selects certain MSC subtypes. Our data clearly show that expansion culture conditions have a significant effect on distribution of a number of MSC surface markers." @default.
• W2080554920 created "2016-06-24" @default.
• W2080554920 creator A5000157714 @default.
• W2080554920 creator A5026672906 @default.
• W2080554920 creator A5028355843 @default.
• W2080554920 creator A5033557216 @default.
• W2080554920 creator A5069799428 @default.
• W2080554920 creator A5074661041 @default.
• W2080554920 creator A5083477495 @default.
• W2080554920 date "2013-07-21" @default.
• W2080554920 modified "2023-10-14" @default.
• W2080554920 title "<scp>FGF</scp> ‐2 addition during expansion of human bone marrow‐derived stromal cells alters <scp>MSC</scp> surface marker distribution and chondrogenic differentiation potential" @default.
• W2080554920 cites W1574963476 @default.
• W2080554920 cites W1968746947 @default.
• W2080554920 cites W1975037405 @default.
• W2080554920 cites W1977567827 @default.
• W2080554920 cites W1978846261 @default.
• W2080554920 cites W1980867815 @default.
• W2080554920 cites W1982408656 @default.
• W2080554920 cites W1982561969 @default.
• W2080554920 cites W1984956442 @default.
• W2080554920 cites W1985082605 @default.
• W2080554920 cites W1992935708 @default.
• W2080554920 cites W1993811760 @default.
• W2080554920 cites W1998246678 @default.
• W2080554920 cites W2005111784 @default.
• W2080554920 cites W2007507349 @default.
• W2080554920 cites W2009310100 @default.
• W2080554920 cites W2011211864 @default.
• W2080554920 cites W2011799963 @default.
• W2080554920 cites W2021164682 @default.
• W2080554920 cites W2025371271 @default.
• W2080554920 cites W2027284829 @default.
• W2080554920 cites W2027408389 @default.
• W2080554920 cites W2035040282 @default.
• W2080554920 cites W2038043656 @default.
• W2080554920 cites W2044322920 @default.
• W2080554920 cites W2051309186 @default.
• W2080554920 cites W2054727711 @default.
• W2080554920 cites W2056281529 @default.
• W2080554920 cites W2057685900 @default.
• W2080554920 cites W2060437379 @default.
• W2080554920 cites W2063048290 @default.
• W2080554920 cites W2067548843 @default.
• W2080554920 cites W2067731590 @default.
• W2080554920 cites W2070612379 @default.
• W2080554920 cites W2079587671 @default.
• W2080554920 cites W2088606656 @default.
• W2080554920 cites W2092515541 @default.
• W2080554920 cites W2092851661 @default.
• W2080554920 cites W2097161142 @default.
• W2080554920 cites W2103116859 @default.
• W2080554920 cites W2109200717 @default.
• W2080554920 cites W2112540399 @default.
• W2080554920 cites W2113619226 @default.
• W2080554920 cites W2131388270 @default.
• W2080554920 cites W2139636615 @default.
• W2080554920 cites W2144268541 @default.
• W2080554920 cites W2149285411 @default.
• W2080554920 cites W2152899237 @default.
• W2080554920 cites W2155969733 @default.
• W2080554920 cites W2158048826 @default.
• W2080554920 cites W2165917089 @default.
• W2080554920 cites W2417173174 @default.
• W2080554920 doi "https://doi.org/10.1111/cpr.12046" @default.
• W2080554920 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6495732" @default.
• W2080554920 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23869761" @default.
• W2080554920 hasPublicationYear "2013" @default.
• W2080554920 type Work @default.
• W2080554920 sameAs 2080554920 @default.
• W2080554920 citedByCount "53" @default.
• W2080554920 countsByYear W20805549202014 @default.
• W2080554920 countsByYear W20805549202015 @default.
• W2080554920 countsByYear W20805549202016 @default.
• W2080554920 countsByYear W20805549202017 @default.
• W2080554920 countsByYear W20805549202018 @default.
• W2080554920 countsByYear W20805549202019 @default.
• W2080554920 countsByYear W20805549202020 @default.
• W2080554920 countsByYear W20805549202021 @default.
• W2080554920 countsByYear W20805549202022 @default.
• W2080554920 countsByYear W20805549202023 @default.
• W2080554920 crossrefType "journal-article" @default.
• W2080554920 hasAuthorship W2080554920A5000157714 @default.
• W2080554920 hasAuthorship W2080554920A5026672906 @default.
• W2080554920 hasAuthorship W2080554920A5028355843 @default.
• W2080554920 hasAuthorship W2080554920A5033557216 @default.
• W2080554920 hasAuthorship W2080554920A5069799428 @default.
• W2080554920 hasAuthorship W2080554920A5074661041 @default.
• W2080554920 hasAuthorship W2080554920A5083477495 @default.
• W2080554920 hasBestOaLocation W20805549202 @default.
• W2080554920 hasConcept C116716535 @default.
• W2080554920 hasConcept C122927707 @default.
• W2080554920 hasConcept C1491633281 @default.
• W2080554920 hasConcept C16685009 @default.
• W2080554920 hasConcept C16930146 @default.
• W2080554920 hasConcept C170493617 @default.
• W2080554920 hasConcept C185592680 @default.
• W2080554920 hasConcept C198826908 @default.

• next