Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080600105> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2080600105 endingPage "30" @default.
- W2080600105 startingPage "224" @default.
- W2080600105 abstract "To establish a simplified, nonradioactive approach for identifying mRNAs on Northern blots, antisense oligonucleotides have been used as probes in combination with chemiluminescence-based detection. Oligonucleotides (approximately 32-mer) were end-labeled with digoxigenin (DIG) and used in conjunction with adamantyl 1,2-dioxetane aryl phosphate substrates (Lumigen PPD and CSPD). Oligonucleotides were designed as probes for several mRNAs in tissues of rats and mice, including the mitochondrial uncoupling protein, lipoprotein lipase, GLUT1, GLUT4, and beta-actin. Uncoupling protein mRNA was detected in total RNA from brown adipose tissue with a 32-mer DIG-labeled oligonucleotide, within 2 min of exposure to film. This mRNA could also be detected when as little as 250 ng of total RNA was applied to the gel, following 4 h exposure to film, and was present only in brown fat. The mRNA for lipoprotein lipase was detectable with a 30-mer DIG-labeled oligonucleotide in 1 micrograms of total RNA from mouse heart, within 2 h of exposure. The mRNA for the GLUT1 glucose transporter was detected in total RNA from rat midbrain using a 32-mer DIG-labeled oligonucleotide, while beta-actin mRNA was detected with a 30-mer oligonucleotide. The mRNA for the insulin-sensitive glucose transporter GLUT4 was detected with a 32-mer DIG-labeled oligonucleotide and found only in those tissues in which glucose uptake is stimulated by insulin. The speed of detection was greater with CSPD and was augmented by exposure of membranes to film at 37 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2080600105 created "2016-06-24" @default.
- W2080600105 creator A5016686335 @default.
- W2080600105 creator A5023985566 @default.
- W2080600105 creator A5038164852 @default.
- W2080600105 creator A5053821871 @default.
- W2080600105 creator A5056226225 @default.
- W2080600105 date "1994-10-01" @default.
- W2080600105 modified "2023-09-25" @default.
- W2080600105 title "Chemiluminescent detection of mRNAs on northern blots with digoxigenin end-labeled oligonucleotides." @default.
- W2080600105 doi "https://doi.org/10.1006/abio.1994.1477" @default.
- W2080600105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7856853" @default.
- W2080600105 hasPublicationYear "1994" @default.
- W2080600105 type Work @default.
- W2080600105 sameAs 2080600105 @default.
- W2080600105 citedByCount "48" @default.
- W2080600105 countsByYear W20806001052013 @default.
- W2080600105 countsByYear W20806001052014 @default.
- W2080600105 crossrefType "journal-article" @default.
- W2080600105 hasAuthorship W2080600105A5016686335 @default.
- W2080600105 hasAuthorship W2080600105A5023985566 @default.
- W2080600105 hasAuthorship W2080600105A5038164852 @default.
- W2080600105 hasAuthorship W2080600105A5053821871 @default.
- W2080600105 hasAuthorship W2080600105A5056226225 @default.
- W2080600105 hasConcept C104317684 @default.
- W2080600105 hasConcept C105580179 @default.
- W2080600105 hasConcept C111824103 @default.
- W2080600105 hasConcept C129312508 @default.
- W2080600105 hasConcept C138626823 @default.
- W2080600105 hasConcept C153911025 @default.
- W2080600105 hasConcept C177707886 @default.
- W2080600105 hasConcept C185592680 @default.
- W2080600105 hasConcept C2776188179 @default.
- W2080600105 hasConcept C2776552150 @default.
- W2080600105 hasConcept C55493867 @default.
- W2080600105 hasConcept C67705224 @default.
- W2080600105 hasConcept C81439078 @default.
- W2080600105 hasConcept C85265743 @default.
- W2080600105 hasConcept C86803240 @default.
- W2080600105 hasConceptScore W2080600105C104317684 @default.
- W2080600105 hasConceptScore W2080600105C105580179 @default.
- W2080600105 hasConceptScore W2080600105C111824103 @default.
- W2080600105 hasConceptScore W2080600105C129312508 @default.
- W2080600105 hasConceptScore W2080600105C138626823 @default.
- W2080600105 hasConceptScore W2080600105C153911025 @default.
- W2080600105 hasConceptScore W2080600105C177707886 @default.
- W2080600105 hasConceptScore W2080600105C185592680 @default.
- W2080600105 hasConceptScore W2080600105C2776188179 @default.
- W2080600105 hasConceptScore W2080600105C2776552150 @default.
- W2080600105 hasConceptScore W2080600105C55493867 @default.
- W2080600105 hasConceptScore W2080600105C67705224 @default.
- W2080600105 hasConceptScore W2080600105C81439078 @default.
- W2080600105 hasConceptScore W2080600105C85265743 @default.
- W2080600105 hasConceptScore W2080600105C86803240 @default.
- W2080600105 hasIssue "1" @default.
- W2080600105 hasLocation W20806001051 @default.
- W2080600105 hasLocation W20806001052 @default.
- W2080600105 hasOpenAccess W2080600105 @default.
- W2080600105 hasPrimaryLocation W20806001051 @default.
- W2080600105 hasRelatedWork W1975128610 @default.
- W2080600105 hasRelatedWork W2021712506 @default.
- W2080600105 hasRelatedWork W2034036767 @default.
- W2080600105 hasRelatedWork W2075920644 @default.
- W2080600105 hasRelatedWork W2080600105 @default.
- W2080600105 hasRelatedWork W2187472444 @default.
- W2080600105 hasRelatedWork W2279637311 @default.
- W2080600105 hasRelatedWork W2420452085 @default.
- W2080600105 hasRelatedWork W2433569996 @default.
- W2080600105 hasRelatedWork W2462257457 @default.
- W2080600105 hasVolume "222" @default.
- W2080600105 isParatext "false" @default.
- W2080600105 isRetracted "false" @default.
- W2080600105 magId "2080600105" @default.
- W2080600105 workType "article" @default.