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- W2080632927 abstract "A simple direct bridge homologous microtitre-plate enzyme immunoassay (EIA) of progesterone in bovine milk is described using a murine anti-progesterone monoclonal antibody raised against 11α-hydroxyprogesterone-11-hemisuccinate bovine serum albumin as the binding agent, horseradish peroxidase as the enzyme label, and tetramethylbenzidine as the chromogen. The standard curve ranged from 0 to 150 pg per well, and the corresponding range of optical density values was 1.86-0.31 measured at 450 nm. The intra- and inter-assay coefficients of variation were less than 10%. The total amount of time needed for the assay of 40 replicate samples was 1.5 h. The influence of milk fat on analytical recovery and on progesterone concentration was investigated. A close negative relationship (r = −0.93) between the recovery and milk fat concentration was found. Progesterone concentrations in whole milk were significantly (P < 0.001) higher than in first milk, but there was no difference in progesterone concentrations measured in whole milk and postmilk strippings, although the milk fat concentration was different (P < 0.001). Comparison with radioimmunoassay based on iodinated tracer and polyclonal antiserum showed good correlation at 0.965. The practicability of the EIA was demonstrated in the selection of pregnant cows with 87.5% precision and non-pregnant cows with 100% precision." @default.
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- W2080632927 date "1993-11-01" @default.
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- W2080632927 title "Enzyme immunoassay (EIA) for milk progesterone using a monoclonal antibody" @default.
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- W2080632927 doi "https://doi.org/10.1016/0378-4320(93)90046-t" @default.
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