Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080641962> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2080641962 abstract "Abstract The mechanism of nucleic acid synthesis and the nature of the nucleic acid products transcribed by the visna virus RNA dependent DNA polymerase have been investigated. Purified virus preparations activated maximally by .05% NP40 detergent catalyzed the linear incorporation of radioactive nucleoside precursor into acid insoluble product for 60 min and at a decreasing rate thereafter. Analysis of nucleic; acid products by S1 nuclease digestion, hydroxylapatite chromatography, and ultracentrifugation in cesium sulfate, indicate that the reaction proceeds in two steps. The initial step is transcription of a single-stranded DNA bound to RNA template. The second step involves synthesis of double-stranded DNA and this reaction can be blocked with actinomycin D. The DNA synthesized in the presence of actinomycin D contains sequences complementary to the entire viral genome. The double-stranded DNA product consists predominantly of a small molecular weight species with a sedimentation coefficient of 4S and an analytical complexity, measured by reassociation kinetics, of approximately 2–4 × 10−3 mole-sec/liter. The visna polymerase therefore preferentially transcribes a limited portion of the viral genome into doublestranded DNA in vitro." @default.
- W2080641962 created "2016-06-24" @default.
- W2080641962 creator A5002178905 @default.
- W2080641962 creator A5023586836 @default.
- W2080641962 creator A5036565288 @default.
- W2080641962 creator A5088084001 @default.
- W2080641962 creator A5089138950 @default.
- W2080641962 date "1974-01-01" @default.
- W2080641962 modified "2023-09-26" @default.
- W2080641962 title "Characterization of the nucleic acid product of the visna virus RNA dependent DNA polymerase" @default.
- W2080641962 cites W1569496200 @default.
- W2080641962 cites W1775749144 @default.
- W2080641962 cites W1795036143 @default.
- W2080641962 cites W1802534420 @default.
- W2080641962 cites W1835778803 @default.
- W2080641962 cites W1932210441 @default.
- W2080641962 cites W1939457734 @default.
- W2080641962 cites W1964309690 @default.
- W2080641962 cites W1979413654 @default.
- W2080641962 cites W1989493272 @default.
- W2080641962 cites W1990234989 @default.
- W2080641962 cites W1994830990 @default.
- W2080641962 cites W201050536 @default.
- W2080641962 cites W2013241878 @default.
- W2080641962 cites W2029035624 @default.
- W2080641962 cites W2032556026 @default.
- W2080641962 cites W2055094603 @default.
- W2080641962 cites W2056935905 @default.
- W2080641962 cites W2074135297 @default.
- W2080641962 cites W2088128491 @default.
- W2080641962 doi "https://doi.org/10.1016/0042-6822(74)90125-1" @default.
- W2080641962 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4131957" @default.
- W2080641962 hasPublicationYear "1974" @default.
- W2080641962 type Work @default.
- W2080641962 sameAs 2080641962 @default.
- W2080641962 citedByCount "14" @default.
- W2080641962 countsByYear W20806419622016 @default.
- W2080641962 countsByYear W20806419622017 @default.
- W2080641962 crossrefType "journal-article" @default.
- W2080641962 hasAuthorship W2080641962A5002178905 @default.
- W2080641962 hasAuthorship W2080641962A5023586836 @default.
- W2080641962 hasAuthorship W2080641962A5036565288 @default.
- W2080641962 hasAuthorship W2080641962A5088084001 @default.
- W2080641962 hasAuthorship W2080641962A5089138950 @default.
- W2080641962 hasConcept C104317684 @default.
- W2080641962 hasConcept C153911025 @default.
- W2080641962 hasConcept C24107716 @default.
- W2080641962 hasConcept C2776449523 @default.
- W2080641962 hasConcept C2777271071 @default.
- W2080641962 hasConcept C54689828 @default.
- W2080641962 hasConcept C54871578 @default.
- W2080641962 hasConcept C552990157 @default.
- W2080641962 hasConcept C55493867 @default.
- W2080641962 hasConcept C67705224 @default.
- W2080641962 hasConcept C82381507 @default.
- W2080641962 hasConcept C86803240 @default.
- W2080641962 hasConceptScore W2080641962C104317684 @default.
- W2080641962 hasConceptScore W2080641962C153911025 @default.
- W2080641962 hasConceptScore W2080641962C24107716 @default.
- W2080641962 hasConceptScore W2080641962C2776449523 @default.
- W2080641962 hasConceptScore W2080641962C2777271071 @default.
- W2080641962 hasConceptScore W2080641962C54689828 @default.
- W2080641962 hasConceptScore W2080641962C54871578 @default.
- W2080641962 hasConceptScore W2080641962C552990157 @default.
- W2080641962 hasConceptScore W2080641962C55493867 @default.
- W2080641962 hasConceptScore W2080641962C67705224 @default.
- W2080641962 hasConceptScore W2080641962C82381507 @default.
- W2080641962 hasConceptScore W2080641962C86803240 @default.
- W2080641962 hasLocation W20806419621 @default.
- W2080641962 hasLocation W20806419622 @default.
- W2080641962 hasOpenAccess W2080641962 @default.
- W2080641962 hasPrimaryLocation W20806419621 @default.
- W2080641962 hasRelatedWork W1963992374 @default.
- W2080641962 hasRelatedWork W1984865072 @default.
- W2080641962 hasRelatedWork W1985254234 @default.
- W2080641962 hasRelatedWork W2034409080 @default.
- W2080641962 hasRelatedWork W2036973195 @default.
- W2080641962 hasRelatedWork W2063208150 @default.
- W2080641962 hasRelatedWork W2065704027 @default.
- W2080641962 hasRelatedWork W2078996111 @default.
- W2080641962 hasRelatedWork W2083193178 @default.
- W2080641962 hasRelatedWork W83043046 @default.
- W2080641962 isParatext "false" @default.
- W2080641962 isRetracted "false" @default.
- W2080641962 magId "2080641962" @default.
- W2080641962 workType "article" @default.