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• W2080644555 abstract "A general strategy for the observation of low γ half-integer quadrupolar nuclides in biological systems is presented. The methodology combines low-temperature (4–100 K) techniques with cross-polarization (CP) experiments while employing a so-called Carr–Purcell–Meiboom–Gill spin-echo sequence (CPMG). This combined approach is termed CP/QCPMG. Also discussed are data processing issues that are unique to the induced signals that result from the QCPMG pulse sequence. Central to this strategy is the development of a stable low-temperature (4 to 250 K) NMR double-resonance probe. The probe is robust enough to handle multiple contact experiments and long acquisition periods with 1H decoupling. This approach is illustrated with low-temperature solid-state 67Zn and 25Mg NMR CP/QCPMG experiments on model compounds. The conclusion reached is that the strategy affords sufficient sensitivity to examine Zn2+ and/or Mg2+ binding sites in metalloproteins." @default.
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• W2080644555 date "2001-07-01" @default.
• W2080644555 modified "2023-10-02" @default.
• W2080644555 title "A General Strategy for the NMR Observation of Half-Integer Quadrupolar Nuclei in Dilute Environments" @default.
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• W2080644555 doi "https://doi.org/10.1006/jmre.2001.2353" @default.
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