FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2080646009> ?p ?o ?g. }

• W2080646009 endingPage "e45" @default.
• W2080646009 startingPage "e44" @default.
• W2080646009 abstract "Introduction Histological analysis of biopsy tissue to assess tissue ingrowth and organization as it populates a novel, biodegradable, polyurethane meniscal scaffold designed for the treatment of painful irreparable partial meniscus tears and/or partial meniscal tissue loss. Methods This was a prospective, single-arm, multicentre clinical investigation in patients with an irreparable partial medial or lateral meniscal tear or partial meniscus loss. The scaffold is designed to support the body's own physiological pathways of tissue repair by providing a temporary three-dimensional matrix for cellular attachment and vascular ingrowth. A biopsy was obtained at 12 months (±1 month) after implantation of the Actifit scaffold in the 44 patients who had agreed to a relook arthroscopy with a biopsy. All biopsies were taken with an arthroscopic punch from the center of the inner free edge of the implanted scaffold meniscus according to a standardized protocol and fixed in 4%-buffered formol for at least 24 hours. Histochemical staining of the biopsy and cellular markers were used to enable visualization of specific cellular and extracellular structures. Results A total of, 52 patients (34 medial: 18 lateral) were treated with the polyurethane meniscal scaffold. The population recruited into the study were young (mean age, 30.8±9.4 years), and the majority (88%) had undergone 1 or 2 prior surgeries on the involved meniscus. The mean longitudinal length of the meniscal defect was 47.1±10.0 mm. All 44 biopsies showed fully vital material, with no signs of necrosis or cell death, illustrating biocompatibility and successful tissue ingrowth into the scaffold meniscus within the knee joint. Notably, in addition to a fibrous capsule, three distinctly different layers were observed: Layer 1: a superficial fibrotic vascularized, tissue layer, mainly consisting of fibroblasts and some fusiform fibrochondroblast-like cells. Layer 2: an avascular “transition” layer situated between the superficial “layer 1” and the deep “layer 3.” Layer 2 consists of a mixture of cell types, of which fusiform cells with a morphology resembling fibrochondroblasts are most prominent. In some biopsies cells with a morphology resembling fibrochondroblasts of the polygonal subtype are prominent. The extracellular matrix is loosely organized with positive staining for collagen type I. Layer 3: a deep, avascular hypocellular layer consisting of cells with a morphology resembling fibrochondroblasts of the polygonal subtype with a matrix rich in fibrin. Conclusion No signs of tissue necrosis or cell death were observed and all biopsies showed complete re-population by cells and tissue that can be regarded as vital structures, illustrating the biocompatibility of the polyurethane scaffold. Importantly, the layered organization of biopsied scaffold meniscus, each with its own histological characteristics, is illustrative of an ongoing process of regeneration, maturation, and remodeling. Moreover, the above-described structures and their organization are similar to that of the native human meniscus. (Actifit Study Group: R. Verdonk, P. Beaufils, J. Bellemans, P. Colombet, R. Cugat, P. Djian, H. Laprell, P. Neyret, H. Paessler.) Histological analysis of biopsy tissue to assess tissue ingrowth and organization as it populates a novel, biodegradable, polyurethane meniscal scaffold designed for the treatment of painful irreparable partial meniscus tears and/or partial meniscal tissue loss. This was a prospective, single-arm, multicentre clinical investigation in patients with an irreparable partial medial or lateral meniscal tear or partial meniscus loss. The scaffold is designed to support the body's own physiological pathways of tissue repair by providing a temporary three-dimensional matrix for cellular attachment and vascular ingrowth. A biopsy was obtained at 12 months (±1 month) after implantation of the Actifit scaffold in the 44 patients who had agreed to a relook arthroscopy with a biopsy. All biopsies were taken with an arthroscopic punch from the center of the inner free edge of the implanted scaffold meniscus according to a standardized protocol and fixed in 4%-buffered formol for at least 24 hours. Histochemical staining of the biopsy and cellular markers were used to enable visualization of specific cellular and extracellular structures. A total of, 52 patients (34 medial: 18 lateral) were treated with the polyurethane meniscal scaffold. The population recruited into the study were young (mean age, 30.8±9.4 years), and the majority (88%) had undergone 1 or 2 prior surgeries on the involved meniscus. The mean longitudinal length of the meniscal defect was 47.1±10.0 mm. All 44 biopsies showed fully vital material, with no signs of necrosis or cell death, illustrating biocompatibility and successful tissue ingrowth into the scaffold meniscus within the knee joint. Notably, in addition to a fibrous capsule, three distinctly different layers were observed: Layer 1: a superficial fibrotic vascularized, tissue layer, mainly consisting of fibroblasts and some fusiform fibrochondroblast-like cells. Layer 2: an avascular “transition” layer situated between the superficial “layer 1” and the deep “layer 3.” Layer 2 consists of a mixture of cell types, of which fusiform cells with a morphology resembling fibrochondroblasts are most prominent. In some biopsies cells with a morphology resembling fibrochondroblasts of the polygonal subtype are prominent. The extracellular matrix is loosely organized with positive staining for collagen type I. Layer 3: a deep, avascular hypocellular layer consisting of cells with a morphology resembling fibrochondroblasts of the polygonal subtype with a matrix rich in fibrin. No signs of tissue necrosis or cell death were observed and all biopsies showed complete re-population by cells and tissue that can be regarded as vital structures, illustrating the biocompatibility of the polyurethane scaffold. Importantly, the layered organization of biopsied scaffold meniscus, each with its own histological characteristics, is illustrative of an ongoing process of regeneration, maturation, and remodeling. Moreover, the above-described structures and their organization are similar to that of the native human meniscus. (Actifit Study Group: R. Verdonk, P. Beaufils, J. Bellemans, P. Colombet, R. Cugat, P. Djian, H. Laprell, P. Neyret, H. Paessler.)" @default.
• W2080646009 created "2016-06-24" @default.
• W2080646009 creator A5043731157 @default.
• W2080646009 creator A5059707718 @default.
• W2080646009 date "2011-05-01" @default.
• W2080646009 modified "2023-09-26" @default.
• W2080646009 title "Histological Analysis of Tissue Ingrowth and Organization Following Implantation of a Novel Synthetic Acellular Meniscal Scaffold for the Treatment of Irreparable Partial Meniscus Tears and/or Partial (SS-27A)" @default.
• W2080646009 doi "https://doi.org/10.1016/j.arthro.2011.03.031" @default.
• W2080646009 hasPublicationYear "2011" @default.
• W2080646009 type Work @default.
• W2080646009 sameAs 2080646009 @default.
• W2080646009 citedByCount "4" @default.
• W2080646009 countsByYear W20806460092014 @default.
• W2080646009 countsByYear W20806460092019 @default.
• W2080646009 countsByYear W20806460092021 @default.
• W2080646009 crossrefType "journal-article" @default.
• W2080646009 hasAuthorship W2080646009A5043731157 @default.
• W2080646009 hasAuthorship W2080646009A5059707718 @default.
• W2080646009 hasConcept C120665830 @default.
• W2080646009 hasConcept C121332964 @default.
• W2080646009 hasConcept C136229726 @default.
• W2080646009 hasConcept C141071460 @default.
• W2080646009 hasConcept C142724271 @default.
• W2080646009 hasConcept C189178095 @default.
• W2080646009 hasConcept C204787440 @default.
• W2080646009 hasConcept C2775934546 @default.
• W2080646009 hasConcept C2776164576 @default.
• W2080646009 hasConcept C2778275304 @default.
• W2080646009 hasConcept C2779162959 @default.
• W2080646009 hasConcept C2780423099 @default.
• W2080646009 hasConcept C2781218938 @default.
• W2080646009 hasConcept C3020332539 @default.
• W2080646009 hasConcept C61511704 @default.
• W2080646009 hasConcept C71924100 @default.
• W2080646009 hasConcept C89429830 @default.
• W2080646009 hasConceptScore W2080646009C120665830 @default.
• W2080646009 hasConceptScore W2080646009C121332964 @default.
• W2080646009 hasConceptScore W2080646009C136229726 @default.
• W2080646009 hasConceptScore W2080646009C141071460 @default.
• W2080646009 hasConceptScore W2080646009C142724271 @default.
• W2080646009 hasConceptScore W2080646009C189178095 @default.
• W2080646009 hasConceptScore W2080646009C204787440 @default.
• W2080646009 hasConceptScore W2080646009C2775934546 @default.
• W2080646009 hasConceptScore W2080646009C2776164576 @default.
• W2080646009 hasConceptScore W2080646009C2778275304 @default.
• W2080646009 hasConceptScore W2080646009C2779162959 @default.
• W2080646009 hasConceptScore W2080646009C2780423099 @default.
• W2080646009 hasConceptScore W2080646009C2781218938 @default.
• W2080646009 hasConceptScore W2080646009C3020332539 @default.
• W2080646009 hasConceptScore W2080646009C61511704 @default.
• W2080646009 hasConceptScore W2080646009C71924100 @default.
• W2080646009 hasConceptScore W2080646009C89429830 @default.
• W2080646009 hasIssue "5" @default.
• W2080646009 hasLocation W20806460091 @default.
• W2080646009 hasOpenAccess W2080646009 @default.
• W2080646009 hasPrimaryLocation W20806460091 @default.
• W2080646009 hasRelatedWork W1968347926 @default.
• W2080646009 hasRelatedWork W1978959252 @default.
• W2080646009 hasRelatedWork W2000098799 @default.
• W2080646009 hasRelatedWork W2074374612 @default.
• W2080646009 hasRelatedWork W2080646009 @default.
• W2080646009 hasRelatedWork W2085429121 @default.
• W2080646009 hasRelatedWork W2103892025 @default.
• W2080646009 hasRelatedWork W2153727764 @default.
• W2080646009 hasRelatedWork W2987318007 @default.
• W2080646009 hasRelatedWork W3128939922 @default.
• W2080646009 hasVolume "27" @default.
• W2080646009 isParatext "false" @default.
• W2080646009 isRetracted "false" @default.
• W2080646009 magId "2080646009" @default.
• W2080646009 workType "article" @default.