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• W2080655949 endingPage "2130" @default.
• W2080655949 startingPage "2121" @default.
• W2080655949 abstract "Aromatic amino acids of membrane proteins are enriched at the lipid-water interface. The role of tryptophan on the folding and stability of an integral membrane protein is investigated with ultraviolet resonance Raman and fluorescence spectroscopy. We investigate a model system, the β-barrel outer membrane protein A (OmpA), and focus on interfacial tryptophan residues oriented toward the lipid bilayer (trp-7, trp-170, or trp-15) or the interior of the β-barrel pore (trp-102). OmpA mutants with a single tryptophan residue at a nonnative position 170 (Trp-170) or a native position 7 (Trp-7) exhibit the greatest stability, with Gibbs free energies of unfolding in the absence of denaturant of 9.4 and 6.7 kcal/mol, respectively. These mutants are more stable than the tryptophan-free OmpA mutant, which exhibits a free energy of unfolding of 2.6 kcal/mol. Ultraviolet resonance Raman spectra of Trp-170 and Trp-7 reveal evolution of a hydrogen bond in a nonpolar environment during the folding reaction, evidenced by systematic shifts in hydrophobicity and hydrogen bond markers. These observations suggest that the hydrogen bond acceptor is the lipid acyl carbonyl group, and this interaction contributes significantly to membrane protein stabilization. Other spectral changes are observed for a tryptophan residue at position 15, and these modifications are attributed to development of a tryptophan-lipid cation-π interaction that is more stabilizing than an intraprotein hydrogen bond by ∼2 kcal/mol. As expected, there is no evidence for lipid-protein interactions for the tryptophan residue oriented toward the interior of the β-barrel pore. These results highlight the significance of lipid-protein interactions, and indicate that the bilayer provides more than a hydrophobic environment for membrane protein folding. Instead, a paradigm of lipid-assisted membrane protein folding and stabilization must be adopted." @default.
• W2080655949 created "2016-06-24" @default.
• W2080655949 creator A5017404890 @default.
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• W2080655949 date "2011-05-01" @default.
• W2080655949 modified "2023-10-11" @default.
• W2080655949 title "Tryptophan-Lipid Interactions in Membrane Protein Folding Probed by Ultraviolet Resonance Raman and Fluorescence Spectroscopy" @default.
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• W2080655949 cites W1964279507 @default.
• W2080655949 cites W1965802152 @default.
• W2080655949 cites W1969651682 @default.
• W2080655949 cites W1971583422 @default.
• W2080655949 cites W1974061371 @default.
• W2080655949 cites W1974207234 @default.
• W2080655949 cites W1974678305 @default.
• W2080655949 cites W1975542665 @default.
• W2080655949 cites W1976747271 @default.
• W2080655949 cites W1976946487 @default.
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• W2080655949 cites W1990122271 @default.
• W2080655949 cites W1993520148 @default.
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• W2080655949 cites W2010499374 @default.
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• W2080655949 cites W2022171111 @default.
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• W2080655949 cites W2025859600 @default.
• W2080655949 cites W2033693106 @default.
• W2080655949 cites W2035211954 @default.
• W2080655949 cites W2041256732 @default.
• W2080655949 cites W2042473787 @default.
• W2080655949 cites W2044692297 @default.
• W2080655949 cites W2047184830 @default.
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• W2080655949 cites W2069572831 @default.
• W2080655949 cites W2069888185 @default.
• W2080655949 cites W2071483422 @default.
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• W2080655949 cites W2104534420 @default.
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• W2080655949 doi "https://doi.org/10.1016/j.bpj.2011.03.018" @default.
• W2080655949 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3149241" @default.
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• W2080655949 hasPublicationYear "2011" @default.
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